Fluoksetin-HCl’nin Kılıçkuyruk Balıklarında (Xiphophorus hellerii) Oluşturduğu Oksidatif Hasarın Belirlenmesi

Bu çalışma ile dünyada gittikçe artış gösteren sağlık sorunlarından biri olan depresyonun tedavisinde yaygın olarak kullanılan ve kardiyovasküler olarak güvenilir kabul edilen SSRI (Özgül Serotonin Geri Alım Engelleyicileri) grubu antidepresanlardan Prozac®’ın etken maddesi olan Fluoksetin Hidroklorür’ün, sucul bir organizma olan kılıçkuyruk balığı (Xiphophorus hellerii Heckel, 1848) dokularında oluşturduğu oksidatif stresin belirlenmesi amaçlanmıştır. Fluoksetin-HCl, doğada yüzey sularında 0.012 μg/L, atık sularda 0.54- 0.929 μg/L doz aralığında bulunmuştur. Bu bilgiler doğrultusunda kılıçkuyruk balıklarına 0.1 μg/L ve 1 μg/L Fluoksetin-HCl uygulaması yapılmıştır. 96 saat sonunda balıklardan kalp ve karaciğer dokuları antiseptik şartlarda disekte edilip homojenize edilmiştir. Daha sonra malondialdehit (MDA), total glutatyon (GSH) miktarları, katalaz (CAT) enzim aktivitesi, süperoksit dismutaz (SOD) enzim aktivitesi ve total protein miktarı spektrofotometrik yöntemlerle belirlenmiştir. Sonuç olarak, kalp dokuda CAT enzim aktivitesi ve MDA seviyesi azalırken, SOD enzim aktivitesi ve GSH seviyesi artmıştır. Karaciğer dokuda ise, CAT enzim aktivitesi ve GSH miktarı artarken, SOD enzim aktivitesi ve MDA seviyesi azalmıştır. Sonuç olarak bu çalışmada kontrol grubu ile yapılan karşılaştırmalar sonucu Fluoksetin-HCl’nin kılıçkuyruk balıklarında stres yolaklarını etkileyerek, stres cevabının düzenlenmesinde etkili olduğu belirlenmiştir.

Anahtar Kelimeler:

Karaciğer

The determination of oxidative damage caused by fluoxetine hydrocloride in swordtail fish (Xiphophorus hellerii)

In this study, it was aimed to determine the oxidative stress in the tissues of the swordtail fish (Xiphophorus hellerii Heckel, 1848) after exposed to the active ingredient of Prozac® and one of the SSRI (Selective Serotonine Reuptake Inhibitor) group antidepressants, Fluoxetine Hydrochloride, which is considered to be safe cardiovascular. It is widely used in the treatment of depression, which is one of the increasing health problems in the World. Fluoxetine-HCl has been found 0.012 μg/L in surface waters and in the dose range of 0.54-0.929 μg/L in wastewater (Sehonova et al., 2018). In line with this information, 0.1 μg / L and 1 μg / L Fluoxetine-HCl was administered to swordtails. At the end of 96 hours, heart and liver tissues of the fish were dissected under antiseptic conditions and homogenized. Later, malondialdehyde (MDA), total glutathione (GSH), catalase (CAT) enzyme activity, superoxide dismutase (SOD) enzyme activity and total protein amount were determined by spectrophotometric methods. As a result, while CAT enzyme activity and MDA level decreased in heart tissue, SOD enzyme activity and GSH level increased. In liver tissue, while CAT enzyme activity and GSH amount increased, SOD enzyme activity and MDA level decreased. As a result of the comparisons with the control group, it was determined that Fluoxetine-HCl is effective in regulating the stress response by affecting the stress pathways in swordtails.

Keywords:

Liver,

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