Antik DNA Çalışmaları ve Karşılaşılan Sorunlar

İleri derecede zarar görmüş ve DNA eldesi için özel koşullarda saklanmamış biyolojik örneklerden DNA elde edilerek gerçekleştirilen moleküler genetik çalışmaları “antik DNA çalışmaları” olarak adlandırılır. Moleküler biyoloji alanında ortaya konulan gelişmeler paralelinde pek çok bilimsel soruna ışık tutacak olan antik DNA çalışmaları, uygulamada standart moleküler genetik çalışmalarına kıyasla farklı hassasiyetler gerektirmektedir. Örneklerin elde edilmesinden laboratuvarda gerçekleştirilen birçok aşamaya kadar pek çok farklı bilim dalından insanın ortak çalışmasını gerektiren bu araştırmalar, sonunda evrimsel ve antropolojik sorulara çözüm sunacak güçtedir. Bu makalede antik DNA çalışmalarının önemi ve çalışma süresince karşılaşılan sorunlar ve çözüm önerileri üzerinde durulacaktır

Ancient DNA

DNA studies from highly degraded biological samples that are not preserved in suitable conditions for DNA analyses are called “ancient DNA analyses” Ancient DNA studies may shed light on many scientific issues in parallel with the improvements of the field of molecular biology, however it requires a higher level of sensitivity during applications when compared to standard molecular genetic studies. These researches raises the need for a collaborative work of scientists from different disciplines to carry out several explorative stages; initiating from sample collection to laboratory analyses which finally provides solutions on the evolutionary and anthropological hypothesis. The aim of this article is to focus on the importance of ancient DNA studies, discuss the problems encountered during these studies and provide solution proposals to overcome these problems

Keywords:

ancient DNA degradation, contamination,

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