Vitamin E plays a protective role while acrylamide administration disrupted the placenta structure in pregnancy: An experimental study

AbstractAim: The present study aimed to investigate possible variations in the placental tissues of rats upon acrylamide (AA) and vitamin E (Vit E) applications between the 0th and 20th days of pregnancy.Materials and Methods: Pregnant rats were divided into control, corn oil, AA, Vit E, AA + Vit E groups. At the end of the experimental period, the abdominal tissue of pregnant rats that were anesthetized with xylazine-ketamine was incised, and the placental tissues that connect the mother and the fetus were removed. Biochemical analyses were conducted based on the malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), protein, total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI) levels in placental tissues.Results: It was observed that there were no differences between the control and corn oil groups. It was determined that AA administration increased MDA, TOS and OSI levels and decreased SOD, CAT, TAS, and GSH levels when compared to all other groups. Vit E administration increased GSH, TAS, SOD and CAT levels when compared to all other groups. In the AA + Vit E group, it was observed oxidative stress parameters approached control group levels.Conclusion: Administration of 10 mg/kg/bw AA led to placental oxidative stress in pregnant rats. This action induced by AA was not only through the increase in MDA, TOS and OSI levels, but also through the reduction of GSH, SOD, CAT, and TAS levels, leading to the deterioration of the oxidant-antioxidant balance. Administration of 100 mg/kg/bw Vit E increased antioxidant capacity in placental tissue. To minimize AA-induced toxicity exposure, we recommend the consumption of sufficient Vit E throughout life not only during pregnancy.

PDF

___

1. Kopp EK, Dekant W. Toxicokinetics of acrylamide in rats and humans following single oral administration of low doses, Toxicol Appl Pharmacol 2009;235:135– 42.
2. Exon JH. A review of the toxicology of acrylamide, J Toxicol Environ Health B Crit Rev. 2006;9:397–412.
3. Parzefall W. Mini review on the toxicity of dietary acrylamide. Food Chem Toxicol 2008;46:1360–4.
4. Edwards PM. The insensitivity of the developing rat foetus to the toxic effects of acrylamide. Chem Biol Interact 1976;12:13-8.
5. LoPachin RM. The changing view of acrylamide neurotoxicity. Neuro Toxicol 2004;25:617–30.
6. Erdemli ME, Aksungur Z, Gul M, et al. The effects of acrylamide and vitamin E on kidneys in pregnancy: an experimental study. J Matern Fetal Neonatal Med 2019;22:3747–56.
7. Erdemli Z, Erdemli ME, Turkoz Y, et al. The effects of acrylamide and Vitamin E administration during pregnancy on adult rats testis. Andrologia 2019;51:e13292.
8. Nordin AM, Walum E, Kjellstrand P, et al. Acrylamide – inducedd effects on general and neurospecific cellular functions during exposure and recovery. Cell Biology and Toxicology 2003;19:43-51. 9.
9. El-Sayyad HI, Abou-Egla MH, El-Sayyad FI, et al. Effects of fried potato chip supplementation on mouse pregnancy and fetal development. Nutr 2011;27:343– 350.
10. Allam A, El-Ghareeb AA, Abdul-Hamid M, et al. Prenatal and perinatal acrylamide disrupts the development of cerebellum in rat: Biochemical and morphological studies, Toxicol Ind Health 2011;27:291–6.
11. El-Sayyad HI, El-Gammal HL, Habak LA, et al. Structural and ultrastructural evidence of neurotoxic effects of fried potato chips on rat postnatal development. Nutr 2011;27:1066-75.
12. Muralidhara SNP. Mitigation of acrylamide-induced behavioral deficits, oxidative impairments and neurotoxicity by oral supplements of geraniol (a monoterpene) in a rat model. Chem-biol Interact 2014;223:27–37.
13. Azzi A, Boscoboinik D, Fazzio A, et al. RRR-alphatocopherol regulation of gene transcription in response to the cell oxidant status. Z Ernahrungswiss 1998;37:21-8.
14. Reiter E, Jiang Q, Christen S. Anti-inflammatory properties of alpha- and gamma-tocopherol. Mol Aspects Med 2007;28:668–91.
15. Naito Y, Shimozawa M, Kuroda M, et al. Tocotrienols reduce 25- hydroxycholesterol - induced monoendothelial cell interaction by inhibiting the surface expression of adhesion molecules. Atherosclerosis 2005;180:19– 25.
16. Tyl RW, Friedman MA, Losco PE, et al. Rat twogeneration reproduction and dominant lethal study of acrylamide in drinking water. Reprod Toxicol 2000;14:385–401.
17. Mazhar MF, Moawad MK, El-Dakdoky HM, et al. Fetotoxicity of 2,4-dichlorophenoxyacetic acid in rats and the protective role of vitamin E. Toxicol Ind Health, 2014;30:480–8.
18. Uchiyama M, Mihara M. Determination of MDA precursor in tissue by TBA test. Anal Biochem 1978;36:271-8.
19. Elman GL. Tissue sulphydryl groups. Arch Biochem Biophys 1979;95:351–8.
20. Jolitha AB, Subramanyam MV, Devi SA. Modification by vitamin E and exercise of oxidative stress in regions of aging rat brain: studies on superoxide dismutase isoenzymes and protein oxidation status. Exp Gerontol 2006;41:753–63.
21. Aebi HI, Bergmayer H. Methods of enzymatic analysis. London: Academic Press 1974; 673–77.
22. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265–75.
23. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005;38:1103-11.
24. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem 2004;37:277-85.
25. Zhao M, Liu X, Luo Y, et al. Evaluation of protective effect of freeze-dried strawberry, grape, and blueberry powder on acrylamide toxicity in mice. J Food Sci 2015;80:869-74.
26. Erdemli ME, Turkoz Y, Altinoz E, et al. Investigation of the effects of acrylamide applied during pregnancy on fetal brain development in rats and protective role of the vitamin E. Hum Exp Toxicol 2016;35:1337–44.
27. Krishna G, Muralidhara P. Inulin supplementation during gestation mitigates acrylamide-induced maternal and fetal brain oxidative dysfunctions and neurotoxicity in rats. Neurotoxicol Teratol 2015; 49: 49–58.
28. Yu Dainan, Xie Xingxing, Qiaoa Bo, et al. Gestational exposure to acrylamide inhibits mouse placental development in vivo. Journal of Hazardous Materials 2019;367:160–70.
29. Yonguc GN, Dodurga Y, Adiguzel E, et al. Grape seed extract has superior beneficial effects than vitamin E on oxidative stress and apoptosis in the hippocampus of streptozotocin induced diabetic rats. Gene 2015;555:119–26.
30. Erdemli ME, Altınöz E, Aksungur Z, et al. Gebe Ratlara uygulanan Akrilamid ve E Vitaminin Plasenta Dokusu Üzerine Olası Etkilerinin Araştırılması. TURJAF 2017;5:349-52.