Burcu KEMAL OKATAN, Sami KARAPOLAT, Alaaddin BURAN, Banu KARAPOLAT

The effects of rifampicin on wound healing

Aim: The effects of topical antibiotics on the wound healing are one of the interesting aspects of medicine. The aim of this study isto investigate the effects of topically applied rifampicin on wound healing in skin defects.Material and Methods: Forty-two male adult Sprague Dawley rats were divided into two groups: Group A (n = 21) and Group B (n= 21). Circular full-thickness skin defects were formed in the right thoracoabdominal regions. Group A received once daily localsaline and Group B received once daily rifampicin 1 cc topically applied on the wounds. The defect sizes were photographed at thebaseline, and days 3, 7 and 10 and the reductions in wound sizes were measured. In each group, 7 rats were sacrificed on each ofdays 3, 7 and 10 and their defected region was resected. Specimens were histopathologically evaluated, and scored for inflammatorycells, collagen accumulation, granulation tissue, re-epithelialization, and features of skin defect such as what layers of the skin areaffected by it, its size and whether it involves any abscess-necrosis. The results were statistically analyzed.Results: There was no statistically significant difference between groups in terms of healing rate. Comparison of scores forinflammatory cells and features of skin defect revealed statistically significant differences. Statistically significant results wereobtained for collagen accumulation and granulation tissue formation in both groups. No statistically significant difference wasfound in re-epithelialization between the groups.Conclusion: Topically applied rifampicin in experimentally induced skin defects does not have a positive effect on wound healing.

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1. Erdur B, Serinken M, Kabay B, et al. Traumatic wound care in Emergency department. Meeting report. Eurasian J Emerg Med 2006;5:20-7.
2. Ozkaya NK, Gumus N, Yilmaz S, et al. Effect of topical nitrofurazone application on partial thickness wound healing. Turk Plast Surg 2015;23:113-7.
3. Thapa RK, Diep DB, Tønnesen HH. Topical antimicrobial peptide formulations for wound healing: Current developments and future prospects. Acta Biomater. 2019 Dec 21. pii: S1742-7061(19)30854- 2. doi: 10.1016/j.actbio.2019.12.025. [Epub ahead of print] Review.
4. Gurel MS, Naycı S, Turgut AV, et al. Comparison of the effects of topical fusidic acid and rifamycin on wound healing in rats. Int Wound J 2015;12:106-10.
5. Zomer HD, Trentin AG. Skin wound healing in humans and mice: Challenges in translational research. J Dermatol Sci 2018;90:3-12.
6. Karapolat S, Gezer S, Yildirim U, et al. Prevention of pulmonary complications of pneumoperitoneum in rats. J Cardiothorac Surg 2011;6:14.
7. Harris C, Forget G, Sidhu K, et al. Percentage reduction in wound size. South West Regional Wound Care Framework Initiative: Toolkit. SWLHIN/SWCCAC: London ON. 2011 Available via: http://www. southwesthealthline.ca/healthlibrary_docs/b.9.2a. woundsizereducinstruc.pdf
8. Sorg H, Tilkorn DJ, Hager S, et al. Skin Wound Healing: An Update on the Current Knowledge and Concepts. Eur Surg Res 2017;58:81-94.
9. Parnell LKS, Volk SW. The Evolution of Animal Models in Wound Healing Research: 1993-2017. Adv Wound Care (New Rochelle) 2019;8:692-702.
10. Wang PH, Huang BS, Horng HC, et al. Wound healing. J Chin Med Assoc 2018;81:94-101.
11. Wilgus TA, Roy S, McDaniel JC. Neutrophils and Wound Repair: Positive Actions and Negative Reactions. Adv Wound Care (New Rochelle)2013;2:379-88.
12. Rajan V, Murray RZ. The duplicitous nature of inflammation in wound repair. Wound Practica and Research. 2008;16:122-9.
13. Kim SH, Lee KM, Lee GS, et al. Rifampicin Alleviates Atopic Dermatitis-Like Response in vivo and in vitro. Biomol Ther (Seoul) 2017;25:634-40.
14. Kuralay F, Cavdar Z. A global view to inflammatory mediators. Genel Tıp Derg 2006;16:143-52.
15. Giovanni G, Giovanni P. Do non-steroidal antiinflammatory drugs and COX-2 selective inhibitors have different renal effects? J Nephrol 2002;15:480-8.
16. Zhou S, Salisbury J, Preedy VR, et al. Increased collagen synthesis rate during wound healing in muscle. PLoS One 2013;8:58324.
17. Futagami A, Ishizaki M, Fukuda Y, et al. Wound healing involves induction of cyclooxygenase-2 expression in rat skin. Lab Invest 2002;82:1503-13.
18. Hess CT. Checklist for Successful Wound Healing Outcomes. Adv Skin Wound Care. 2020;33:54-5.
19. Han G, Ceilley R. Chronic Wound Healing: A Review of Current Management and Treatments. Adv Ther 2017;34:599-610.
20. Kahramanca Ş, Kaya O, Azılı C, et al. Does topical rifampicin reduce the risk of surgical field infection in hernia repair? Ulus Cerrahi Derg 2013;29:54-8.
21. Aygün F, Kuzgun A, Ulucan S, et al. The protective effect of topical rifamycin treatment against sternal wound infection in diabetic patients undergoing on-pump coronary artery bypass graft surgery. Cardiovasc J Afr 2014;25:96-9.
22. Saydam IM, Yilmaz S, Seven E. The influence of topically applied nitrofurazone and rifamycin on full thickness wound healing. Cumhuriyet Med J. 2005;27:113-20.
23. Iselin F, Audren JL, Gouet O, et al. Comparative study of the effects of a local antibiotic and a local antiseptic in emergency hand surgery. Ann Chir Main Memb Super. 1990;9:65-71.