The effect of oral puerarin administration on wound healing in diabetic rat model

Aim: The aim of this study is to examined the effect of the oral administration of puerarin on wound healing in diabetic rat.Material and methods: Twenty-four rats were divided into three groups: Group 1 (n:8) non diabetic control (NDC), group 2 (n:8) diabetic control (DC), group 3 (n:8) diabetic puerarine (DP). Rats Group DC and DP were injected a single dose 200 mg/kg streptozin solution for diabet model. A full thickness linear incision was made in the paravertebral region of the rats and the wound edges were covered with a sterile plaster. In Group NDC and DC, rats were given saline solution for 7 days. In Group DP, rats were given 200 mg/kg puerarin with oral gavage for 7 days. All animals were euthanized at 7 days postoperative. Connective tissue volumes and new vessel formation were measured with stereological analysisResults: Stereologic analysis showed that Group NDC and DP had significantly more connective tissue volume at 7 days compared with group DC. New vessel formation volumes were also significantly lesser in group DC. Connective tissue and new vessel volumes difference were not statistically significant between group NDC and DP.Conclusion: Orally administered puerarin enhances new vessel formation and support wound healing in diabetic rats.

PDF

___

Bullard KM, Cowie CC, Lessem SE, et al. Prevalence of Diagnosed Diabetes in Adults by Diabetes Type -United States, 2016. MMWR Morb Mortal Wkly Rep. 2018;67:359-61.
Strain WD, Paldánius PM. Diabetes, cardiovascular disease and the microcirculation. Cardiovasc Diabetol. 2018;17:57.
Andersson L, Andreasen JO. Soft tissue injuries. In: Andreasen JO, Andreasen FM, Andersson L, eds. Textbook and color atlas of traumatic injuries to the teeth, 4th edition. Oxford; Blackwell Munksgaard; 2007. p. 577-97.
Mostafavinia A, Masteri Farahani R, Abdollahifar MA, et al. Evaluation of the effects of photobiomodulation on partial osteotomy in streptozotocin-induced diabetes in rats. Photomed Laser Surg. 2018;31.
Heshmati J, Namazi N. Effects of black seed (Nigella sativa) on metabolic parameters in diabetes mellitus: a systematic review. Complement Ther Med. 2015;23:275-82.
Majid SA, Khorasgani MR, Emami SH, et al. Study of Diabetic cutaneous wound healing in rats treated with Lactobacillus Casei and its Exopolysaccharide. International Journal of Advanced Biotechnology and Research. 2016;7:2083-92.
Zhou YX, Zhang H, Peng C. Puerarin: a review of pharmacological effects. Phytother Res. 2014;28:961-75
Hu W, Yang X, Zhe C, et al. Puerarin inhibits iNOS, COX-2 and CRP expression via suppression of NF-κB activation in LPS-induced RAW264.7 macrophage cells. Pharmacol Rep. 2011;63:781-9.
Zhang W, Liu CQ, Wang PW, et al. Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet. Eur J Pharmacol. 2010;649:398-402.
Gundersen HJ, Jensen EB, Kiêu K, et al. The efficiency of systematic sampling in stereology--reconsidered. J Microsc. 1999;193:199-211.
Kochi G, Sato S, Fukuyama T, et al. Analysis on the guided bone augmentation in the rat calvarium using a microfocus computerized tomography analysis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;107:42-8.
Ritarwan K, Lelo A, Pane YS, et al. Increasing Atherosclerosis in Streptozotocin-Induced Diabetes into Four Groups of Mice. Open Access Maced J Med Sci. 2018;6:287-92.
Mayyas F, Jaradat R, Alzoubi KH. Cardiac effects of fish oil in a rat model of streptozotocin-induced diabetes. Nutr Metab Cardiovasc Dis. 2018;28:592-99.
Deeds MC, Anderson JM, Armstrong AS, et al. Single dose streptozotocin-induced diabetes: considerations for study design in islet transplantation models. Lab Anim. 2011;45:131-40.
Hasegawa H, Ozawa S, Hashimoto K, et al. Type 2 diabetes impairs implant osseointegration capacity in rats. Int J Oral Maxillofac Implants. 2008;23:237-46.
Hong JP, Jung HD, Kim YW. Recombinant human epidermal growth factor (EGF) to enhance healing for diabetic foot ulcers. Ann Plast Surg. 2006;56:394-8.
Galiano RD, Tepper OM, Pelo CR, et al. Topical vascular endothelial growth factor accelerates diabetic wound healing through increased angiogenesis and by mobilizing and recruiting bone marrow-derived cells. Am J Pathol. 2004;164:1935-47.
Marston WA. Dermagraft, a bioengineered human dermal equivalent for the treatment of chronic nonhealing diabetic foot ulcer. Expert Rev Med Devices. 2004;1:21-31.
Micera A, Vigneti E, Pickholtz D, et al. Nerve growth factor displays stimulatory effects on human skin and lung fibroblasts, demonstrating a direct role for this factor in tissue repair. Proc Natl Acad Sci U S A. 2001;98:6162-7.
Al-Numair KS, Chandramohan G, Veeramani C, et al. Ameliorative effect of kaempferol, a flavonoid, on oxidative stress in streptozotocin-induced diabetic rats. Redox Rep. 2015;20:198-209.
Hadjzadeh MA, Rajaei Z, Moradi R, et al. Effects of Hydroalcoholic Extract Of Watercress (Nasturtium Officinale) Leaves On Serum Glucose And Lipid Levels In Diabetic Rats. Indian J Physiol Pharmacol. 2015;59:223-30.
Rocha DS, Casagrande L, Model JFA, et al. Effect of yerba mate (Ilex paraguariensis) extract on the metabolism of diabetic rats. Biomed Pharmacother. 2018;105:370-6.
Li L, Yin H, Zhao Y, et al. Protective role of puerarin on LPS/D-Gal induced acute liver injury via restoring autophagy. Am J Transl Res. 2018;10:957-65.
Yin MS, Zhang YC, Xu SH, et al. Puerarin prevents diabetic cardiomyopathy in vivo and in vitro by inhibition of inflammation. J Asian Nat Prod Res. 2018;11:1-8.
Lee JH, Jeon YD, Lee YM, et al. The suppressive effect of puerarin on atopic dermatitis-like skin lesions through regulation of inflammatory mediators in vitro and in vivo. Biochem Biophys Res Commun. 2018;498:707-14.
Novaes RD, Penitente AR, Talvani A, Use of fluorescence in a modified disector method to estimate the number of myocytes in cardiac tissue. Arq Bras Cardiol. 2012;98:252-8.