Yılmaz GÜLER, İshak Suat ÖVEY, Dilara NEMUTLU SAMUR, Bayhan KARABULUT

Low level laser application reduces the effect of 5 fluorouracil/leucovorin combination on human breast cancer cells

Aim : 5-fluorouracil has been widely used in breast cancer treatment. Low level laser therapy (LLLT) has been shown to modulatebiological processes and used in cancer treatment. It has been reported that apoptosis is induced by stimulation of transient receptorpotential protein channels in numerous cancer cells, including breast cancer. We aimed to reveal the effects of 5-fluorouracil /Leucovorin (5-FU/LV) and . low level laser on apoptosis via transient receptor protein ancyrin 1 (TRPA1) channels.Material and Methods: Breast cancer cells (MCF-7) were cultured and cells were divided into seven main groups. Cells wereincubated with 5-FU/LV and LLLT exposure separately and together performed on MCF-7 cell cultures. Cell cultures incubated withTRPV1 channel antagonist capsazepin and stimulator capsaicin. The effects of 5-FU and LLLT were investigated on all molecularpathways of apoptosis.Results: It was found that the levels of apoptosis in 5-FU/LV group were significantly increased in cancer cells compared to controlgroup .TRPA1 channel stimulator administration resulted in significantly increased apoptosis levels compared to the control group,in 5-FU/LV + low level laser group the apoptosis level significantly reduced compared to 5-FU/LV-only group. (p

PDF

___

1. Devgan M, Karar PK, Agarwal G, et al. In silico designing of drugs for the inhibition of AMF-HER2 complex in trastuzumab resistant breast cancer. Indian J Biotechnol 2016;15:292-8.
2. Wang X, Wang Y, Gu J, et al. ADAM12-L confers acquired 5-fluorouracil resistance in breast cancer cells. Sci Rep 2017;29:9687.
3. de Pauli Paglioni M, Araújo ALD, Arboleda LPA, et al. Tumor safety and side effects of photobiomodulation therapy used for prevention and management of cancer treatment toxicities. A systematic review. Oral Oncol 2019;93:21-8.
4. Khori V, Alizadeh AM, Gheisary Z, et al. The effects of low-level laser irradiation on breast tumor in mice and the expression of Let-7a, miR-155, miR-21, miR125 and miR376b. Lasers Med Sci 2016;31:1775-82.
5. Sonis ST, Hashemi S, Epstein JB, et al. Could the biological robustness of low level laser therapy (photobiomodulation) impact its use in the management of mucositis in head and neck cancer patients. Oral Oncol 2016;54:7-14.
6. Zarei M, Wikramanayake TC, Falto-Aizpurua L, et al. Low level laser therapyand hair regrowth: an evidencebased review. Lasers Med Sci 2016;31:363-71.
7. Moran MM . TRP Channels as Potential Drug Targets. Annu Rev Pharmacol Toxicol 2018;6(58):309-330.
8. 8. Li H. TRP Channel Classification. Adv Exp Med Biol 2017;976:1-8.
9. Takahashi N, Chen HY, Harris IS, et al. Cancer Cells Co-opt the Neuronal Redox-Sensing Channel TRPA1 to Promote Oxidative-Stress Tolerance. Cancer Cell 2018;33:985-1003.
10. Shapovalov G, Ritaine A, Skryma R, et al. Role of TRP ion channels in cancer and tumorigenesis. Semin Immunopathol 2016;38:357-69.
11. Zhao D, Han DF, Wang SS, et al. Roles of tumor necrosis factor-α and interleukin-6 in regulating bone cancer pain via TRPA1 signal pathway and beneficial effects of inhibition of neuro-inflammation and TRPA1. Mol Pain 2019;15:1744806919857981.
12. Han Q, Chen R, Wang F, et al. Pre-exposure to 50 Hzelectromagnetic fields enhanced the antiproliferative efficacy of 5-fluorouracil in breast cancer MCF-7 cells. PLoS One 2018;13:0192888.
13. Martinez NA, Ayala AM, Martinez M, et al. Caveolin-1 Regulates the P2Y2 Receptor Signaling in Human 1321N1 Astrocytoma Cells. J Biol Chem 2016;291:12208-22.
14. Oz A, Celik O. Curcumin inhibits oxidative stressinduced TRPM2 channel activation, calcium ion entry and apoptosis values in SH-SY5Y neuroblastoma cells: Involvement of transfection procedure. Mol Membr Biol 2016;33:76-88.
15. Yigit M, Gunes A, Uguz C, et al. Effects of astaxanthin on antioxidant parameters in ARPE-19 cells on oxidative stress model. Int J Ophthalmol 2019;12:930-5.
16. Behringer EJ, Hakim A. Functional Interaction among KCa and TRP Channels for Cardiovascular Physiology: Modern Perspectives on Aging and Chronic Disease Int J Mol Sci 2019;20:1380.
17. Varghese E, Samuel SM, Sadiq Z, et al. Anti-Cancer Agents in Proliferation and Cell Death: The Calcium Connection. Int J Mol Sci 2019;20:3017.
18. Silva VR, Corrêa RS, de Souza Santos L, et al. A ruthenium-based 5-fluorouracil complex with enhanced cytotoxicity and apoptosis induction action in HCT116 cells. Sci Rep 2018:8:288.
19. Tomankova K, Kolarova H, Vachutka J, et al. Study of photodynamic, sonodynamic and antioxidative influence on HeLa cell line. Indian J Biochem Biophys 2014;51:19-28.
20. Ghosh R, Girigoswami K, Dipanjan G. Suppression of apoptosis leads to cisplatin resistance in V79 cells subjected to chronic oxidative stress. Indian J Biochem Biophys 2012;49:363-70.
21. Feng Y, Gao S, Gao Y, et al. Anti-EGFR antibody sensitizes colorectal cancer stem-like cells to Fluorouracil-induced apoptosis by affecting autophagy. Oncotarget 2016;7:81402-9.
22. Wang T, Chen, Zhu Y, Pan O, et al. Inhibition of Transient Receptor Potential Channel 5 Reverses 5-Fluorouracil Resistance in Human Colorectal Cancer Cells. J Biol Chem 2015;290:448-56.
23. Vancauwenberghe E, Noyer L, Derouiche S, et al. Activation of mutated TRPA1 ion channel by resveratrol in human prostate cancer associated fibroblasts (CAF). Mol Carcinog 2017;56:1851-67.
24. Bayat M, Virdi A, Rezaei F, et al. Comparison of the in vitro effects of low-level laser therapy and lowintensity pulsed ultrasound therapy on bony cells and stem cells. Prog Biophys Mol Biol 2018;133:36-48.
25. Zecha JA, Raber-Durlacher JE, Nair RG, et al. Low level laser therapy/photobiomodulation in the management of side effects of chemoradiation therapy in head and neck cancer: part 1: mechanisms of action, dosimetric, and safety considerations. Support Care Cancer 2016;24:2781-92.
26. Bamps M, Dok R, Nuyts S. Low-Level Laser Therapy Stimulates Proliferation in Head and Neck Squamous Cell Carcinoma Cells. Front Oncol 2018;8:343.
27. Kara C, Selamet H, Gokmenoglu C, et al. Low level laser therapy induces increased viability and proliferation in isolated cancer cells. Cell Prolif 2018;51:12417.
28. Jafarirad S, Hammami Torghabe E, Rasta SH, et al. A novel non-invasive strategy for low-level laserinduced cancer therapy by using new Ag/ZnO and Nd/ZnO functionalized reduced graphene oxide nanocomposites. Artif Cells Nanomed Biotechnol 2018;46:800-16.