Evaluation of dynamic thiol-disulfide balance in children with stage 3-5 chronic kidney disease
Evaluation of dynamic thiol-disulfide balance in children with stage 3-5 chronic kidney disease
Aim: We aimed to examine dynamic thiol-disulfide balance, an indicator of oxidative stress, in children with stage 3-5 chronic kidneydisease (CKD).Material and Methods: Native thiol (Nt), total thiol (Tt) and disulfide (DS) levels were determined spectrophotometrically in 27 childrenwith CKD (14 non-dialysis, 13 hemodialysis) and 40 healthy controls.Results: Albumin, Nt, Tt and DS levels were markedly lower in CKD patients than in controls. However, the DS/Nt values were notstatistically significant between the two groups. When Nt, Tt, DS and DS/Nt values were adjusted for albumin levels, Tt and Ntlevels were lower in CKD patients than controls. In the CKD group, Nt and Tt levels were positively correlated with the albumin andestimated glomerular filtration rate and negatively with urea and creatinine.Conclusion: According to these results, decreased Nt and Tt levels in patients with CKD stages 3–5 may be evidence of exposure tooxidative stress.
___
- 1. Shroff R, Weaver DJ Jr, Mitsnefes MM. Cardiovascular
complications in children with chronic kidney disease. Nat
Rev Nephrol 2011;7:642-9.
- 2. Daenen K, Andries A, Mekahli D, et al. Oxidative stress in
chronic kidney disease. Pediatr Nephrol 2019;34:975-91.
- 3. Matsuyama Y, Terawaki H, Terada T, et al. Albumin thiol
oxidation and serum protein carbonyl formation are
progressively enhanced with advancing stages of chronic
kidney disease. Clin Exp Nephrol 2009;13:308-15.
- 4. Jones DP. Redefining oxidative stress. Antioxid Redox
Signal. 2006;8:1865-79.
- 5. Deneke SM. Thiol-based antioxidants. Curr Top Cell Regul
2000;36:151-80.
- 6. Jones DP, Liang Y. Measuring the poise of thiol/disulfide
couples in vivo. Free Radic Biol Med 2009;47:1329-38.
- 7. Biswas S, Chida AS, Rahman I. Redox modifications of
protein–thiols: emerging roles in cell signaling. Biochem
Pharmacol 2006;71:551-64.
- 8. Circu ML, AwTY. Reactive oxygen species, cellular redox
systems, and apoptosis. Free Radic Biol Med 2010;48:749-
62.
- 9. Erel O, Neselioglu S. A novel and automated assay for thiol/
disulphide homeostasis. Clin Biochem 2014;47:326-32.
- 10. Guzelcicek A, Cakirca G, Erel O, et al. Assessment of thiol/
disulfide balance as an oxidative stress marker in children
with β-thalassemia major. Pak J Med Sci 2019;35:161-5.
- 11. Dirican N, Dirican A, Sen O, et al. Thiol/disulfide homeostasis:
A prognostic biomarker for patients with advanced nonsmall
cell lung cancer? Redox Rep 2016;21:197-203.
- 12. Cakirca G, Celik MM, Erdal H, et al. Investigation of thiol/
disulfide homeostasis in familial mediterranean fever
patients. J Clin Anal Med 2018;9:231-4.
- 13. Gulpamuk B, Tekin K, Sonmez K, et al. The significance of
thiol/disulfide homeostasis and ischemia-modified albumin
levels to assess the oxidative stress in patients with
different stages of diabetes mellitus. Scand J Clin Lab Inv
2018;78:136-42.
- 14. Schwartz GJ, Munoz A, Schneider MF, et al. New equations
to estimate GFR in children with CKD. J Am Soc Nephrol
2009;20:629-37.
- 15. Turell L, Radi R, Alvarez B. The thiol pool in human plasma:
the central contribution of albumin to redox processes. Free
radic Biol Med. 2013;65:244-53.
- 16. Oberg BP, McMenamin E, Lucas FL, et al. Increased
prevalence of oxidant stress and inflammation in patients
with moderate to severe chronic kidney disease. Kidney Int
2004;65:1009-16.
- 17. Dursun E, Ozben T, Suleymanlar G, et al. Effect of hemodialysis
on the oxidative stress and antioxidants. Clin Chem Lab Med
2002;40:1009-13.
- 18. Himmelfarb J, McMonagle E, McMenamin E. Plasma protein
thiol oxidation and carbonyl formation in chronic renal
failure. Kidney Int 2000;58:2571-8.
- 19. Ateş İ, Özkayar N, Yılmaz FM, et al. Oxidative stress level
in patients with chronic kidney disease. Ortadogu Med J
2018;10:45-50.
- 20. Himmelfarb J, McMenamin E, McMonagle E. Plasma
aminothiol oxidation in chronic hemodialysis patients.
Kidney Int 2002;61:705-16.
- 21. Wlodek PJ, Iciek MB, Milkowski A, et al. Various forms of
plasma cysteine and its metabolites in patients undergoing
hemodialysis. Clin Chim Acta 2001;304:9-18.
- 22. Annuk M, Zilmer M, Lind L, et al. Oxidative stress and
endothelial function in chronic renal failure. J Am Soc
Nephrol 2001;12:2747-52.
- 23. Danielski M, Ikizler TA, McMonagle E, et al. Linkage of
hypoalbuminemia, inflammation, and oxidative stress in
patients receiving maintenance hemodialysis therapy. Am J
Kidney Dis 2003;42:286-94.
- 24. Prakash M, Upadhya S, Prabhu R. Protein thiol oxidation and
lipid peroxidation in patients with uraemia. Scand J Clin Lab
Invest 2004;64:599-604.
- 25. Riella MC. Malnutrition in dialysis: malnourishment or
uremic inflammatory response? Kidney Int 2000;57:1211-
32.
- 26. Carrero JJ, Aguilera A, Stenvinkel P, et al. Appetite disorders
in uremia. J Ren Nutr 2008;18:107-13.
- 27. Hsu SP, Chiang CK, Yang SY, et al. N-acetylcysteine for the
management of anemia and oxidative stress in hemodialysis
patients. Nephron Clin Pract 2010;116:207-16.
- 28. Agarwal R, Vasavada N, Sachs NG, et al. Oxidative stress and
renal injury with intravenous iron in patients with chronic
kidney disease. Kidney Int 2004;65:2279-89.