Effects of single high-dose systemic vitamin D injection to dentin-grafted bone defects in osteoporotic rats

Aim: Osteoporosis is a metabolic bone disease posing a public health issue worldwide. Studies showed that dentine can be an alternative bone graft material in bone defects. The purpose of this study is to investigate the potential of the intraperitoneal administration of vitamin D on dentine grafted critical-sized cortical bone defects in osteoporotic rat model.Material and Methods: Twenty-four rats were underwent bilateral ovariectomy and 6 weeks after ovariectomy osteoporotic rats model were obtained and divided into three groups: Group A; Dentine-D vit, Group B; Dentine and Group C (control). A 5-mm diameter critical- size defect was created in the calvarium of each animal. In Group C, untreated control defects. In Group Dentin, defects were filled with particulate human dentine. In Group Dentin-D vit, defects were filled with particulate human dentine and rats were injected intraperitoneally a single high dose (50.000 U.I./kg) vitamin D. All animals were euthanized at 28 days postoperative.Results: New bone formation was evaluated by histologic and immunohistochemical analysis. Histologic analysis showed that Group Dentin-D vit and Dentin had significantly more new bone at 4 weeks compared with group C. According to immunohistochemical analyses, in group Dentin-D vit, BMP2 staining areas and density were statistically higher than Control group. TGF-β level differences between groups Dentin-D vit and Dentin were statistically significant. Group C had a significantly lower TGF-β grade than the other groups. The osteopontin staining areas in group Dentin-D vit were statistically higher grade than in group Dentin and group C. The difference between groups Dentin and C was also statistically significant.Conclusion: Intraperitoneally injected single high dose vitamin D had positive effect on dentine graft and bone formation.

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1. Sweet MG, Sweet JM, Jeremiah MP, et al, Diagnosis and treatment of osteoporosis. Am Fam Physician 2009;79:193-200.
2. Hendrickx G, Boudin E, Van Hul W, A look behind the scenes: the risk and pathogenesis of primary osteoporosis. Nat Rev Rheumatol 2015;11:462-74.
3. Gardner MJ, Demetrakopoulos D, Shindle MK, et al. Osteoporosis and skeletal fractures. HSS J 2006;2:62-9.
4. Zittermann A, Ernst JB, Gummert JF, et al. Vitamin D supplementation body weight and human serum 25-hydroxyvitamin D response: a systematic review. Eur J Nutr 2014;53:367-74.
5. Calori GM, Mazza E, Colombo Met al. The use of bone-graft substitutes in large bone defects: any specific needs? Injury 2011;42:56-63.
6. Neovius E, Engstrand T, Craniofacial reconstruction with bone and biomaterials: review over the last 11 years. J Plast Reconstr Aesthet Surg 2010;63:1615-23.
7. Um IW, Kim YK, Mitsugi M, Demineralized dentin matrix scaffolds for alveolar bone engineering. J Indian Prosthodont Soc 2017;17:120-27.
8. Valdec S, Pasic P, Soltermann A, et al, Alveolar ridge preservation with autologous particulated dentin-a case series. Int J Implant Dent 2017;3:12.
9. Kim SY, Kim YK, Park YH, et al, Evaluation of the Healing Potential of Demineralized Dentin Matrix Fixed with Recombinant Human Bone Morphogenetic Protein-2 in Bone Grafts. Materials (Basel) 2017;10:E1049.
10. Kamal M, Andersson L, Tolba R, et al, Bone regeneration using composite non-demineralized xenogenic dentin with beta-tricalcium phosphate in experimental alveolar cleft repair in a rabbit model. J Transl Med 2017;15:263.
11. Moharamzadeh K, Freeman C, Blackwood K, Processed bovine dentine as a bone substitute, Br J Oral Maxillofac Surg 2008;46:110-3.
12. Tatli U, Ustün Y, Kürkçü M, et al, Effects of zoledronic acid on healing of mandibular fractures: an experimental study in rabbits. J Oral Maxillofac Surg 2011;69:1726-35.
13. Sozmen M, Kabak YB, Gulbahar MY, et al. IImmunohistochemical characterization of peroxisome proliferator-activated receptors in canine normal testis and testicular tumours, J Comp Pathol 2013;149:10-8.
14. Choi JY, Jung UW, Kim CS, et al, The effects of newly formed synthetic peptide on bone regeneration in rat calvarial defects. J Periodontal Implant Sci 2010;40:11-8.
15. Vajgel A, Mardas N, Farias BC, et al. A systematic review on the critical size defect model. Clin Oral Implants Res 2014;25:79-93.
16. Zhong W, Sumita Y, Ohba S, et al: In vivo comparison of the bone regeneration capability of human bone marrow concentrates vs. platelet-rich plasma. PLoS One 2012;7:e40833.
17. Umar M, Sastry KS, Chouchane AI, Role of Vitamin D Beyond the Skeletal Function: A Review of the Molecular and Clinical Studies. Int J Mol Sci 2018;19:E1618.
18. Gaugris S, Heaney RP, Boonen S, et al. Vitamin D inadequacy among post-menopausal women: a systematic review. QJM 2005;98:667-76.
19. Fischer V, Haffner-Luntzer M, Prystaz K, et al, Calcium and vitamin-D deficiency marginally impairs fracture healing but aggravates posttraumatic bone loss in osteoporotic mice. Sci Rep 2017;7:7223.
20. Fentaw Y, Woldie H, Mekonnen S, et al. Change in serum level of vitamin D and associated factors at early phase of bone healing among fractured adult patients at University of Gondar teaching hospital. Northwest Ethiopia: a prospective follow up study. Nutr J 2017;16:54.
21. Song I, Kim BS, Kim CS, et al. Effects of BMP-2 and vitamin D3 on the osteogenic differentiation of adipose stem cells. Biochem Biophys Res Commun 2011;408:126-31.
22. Saad El-Din S, Fouad H, Rashed LA, et al. Impact of Mesenchymal Stem Cells and Vitamin D on Transforming Growth Factor Beta Signaling Pathway in Hepatocellular Carcinoma in Rats. Asian Pac J Cancer Prev 2018;19:905-12.
23. Lau WL, Leaf EM, Hu MC, et al, Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet. Kidney Int 2012;82:1261-70.