Comparison of the effects of variables in sperm preparation techniques on pregnancy rates in intrauterine insemination

Comparison of the effects of variables in sperm preparation techniques on pregnancy rates in intrauterine insemination

Aim: To investigate the effects of two sperm preparation techniques, density gradient centrifugation (DGC) and swim-up, on thepregnancy rates per cycle and per group in two intrauterine (IUI) cycles.Material and Methods: The retrospective study reviewed 634 patients who presented to Firat University Medical School Hospital andunderwent a total of 1,032 IUI cycles. Group I consisted of 306 (48.3%) patients who underwent a total of 524 IUI cycles betweenJanuary 2012 and January 2016 and Group II included 328 (51.7%) patients who underwent a total of 508 IUI cycles between April 2015and January 2019. The study investigated the effects of technical changes applied to sperm preparation techniques on pregnancyrates in both groups.Results:A significant difference was found between the clinical pregnancy rates of the two groups with regard to the numbers ofpatients and IUI cycles (p

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  • 1. Fritz AM, Speroff L. Assisted reproductive technologies. in: clinical gynecologic endocrinology and infertility. 8thth edition. Philadeıphia,: Lippincott Williams 2011;1331-83.
  • 2. Tarlatzis BC, Bontis J, Kolibianakis EM, et al. Mantalenakis S.evaluation of intrauterine insemination with washed spermatozoa from the husband in the treatment of infertility. Hum Reprod 1991;6: 1241-46.
  • 3. Tomlinson MJ, Amissah-Arthur JB, Thompson KA, et al. Prognostic indicators for intrauterine insemination (IUI): statistical model for IUI success. Hum Reprod 1996;11:1892-1896.
  • 4. Rappa KL, Rodriguez HF, Hakkarainen GC, et al. Sperm processing for advanced reproductive technologies: Where are we today? Biotechnol Adv 2016;34:578-87.
  • 5. Paasch U, Grunewald S, Glander HJ. Sperm selection in assisted reproductive techniques. Soc Reprod Fertil Suppl 2007;65:515-25.
  • 6. Özkavukçu S, Aras D. Sperm Preparation Methods for IUI and IVF. TJRMS 2017;1:92-8.
  • 7. Mortimer D. Sperm recovery techniques to maximize fertilizing capacity. Reprod Fertil Def 1994;6:25-31.
  • 8. Agarwal A, Nallella KP, Allamaneni SS, et al. Role of antioxidants in treatment of male infertility: an overview of the literatüre. Reprod Biomed 2004;8:616- 27.
  • 9. O’Flaherty C, Lamirande E, Gagnon C. Positive role of reactive oxygen species in mammalian sperm capacitation: triggering and modulation of phosphorylation events. Free Radic Biol Med 2006;41:528–40.
  • 10. Said TM, Agarwal A, Sharma RK. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 2003;79:829–43.
  • 11. Benchaib M, Braun V, Lornage J, et al.Guerin JF. Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique. Hum Reprod 2003;18:1023-8.
  • 12. Henkel R, Kierspel E, Stalf T, et al. Schill WB, kruger TF. effect of reactive oxygen species produced by spermatozoa and leucocytes on sperm functions in non-leukocytospermic patients. Fertil Steril 2005;83:635-42.
  • 13. Henkel RR, Schill WB. Sperm preparation for ART. Reprod Biol Endocrinol 2003;1:108-29.
  • 14. Enginsu ME, Pieters MH, Dumolin JC, et al. Male factor as determinant of in vitro fertilization outcome. Hum Reprod 1992;7:1136-40.
  • 15. Amiri I, Ghorbani M, Heshmati S. Comparison of the DNA fragmentation and the Sperm parameters after processing by the density gradient and the swim-up methods. J Clin Diagn Res 2012;6:1451-3.
  • 16. Tomlinson MJ, Moffatt O, Manicardi GC, , et al. Interrelationships between seminal parameters and sperm nuclear DNA damage before and after density gradient centrifugation implications for assisted conception. Hum Reprod 2001;16:2160-5.
  • 17. Agarwal A, Said TM. Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum Repro 2003;9:331-45.
  • 18. Aitken RJ, De Iuliis GN. Origins and consequences of DNA damage in male germ cells. Reprod Biomed 2007;14:727-33.
  • 19. Bungum M, Humaidan P, Axmon A, et al.Giwercman A. sperm DNA integrity assessment in prediction of assisted reproduction technology outcome. Hum Reprod 2007;22:174-9.
  • 20. Guzick D, Sullivan M, Adamson GD, et al. Steinkampf MP. Efficacy of treatment for unexplained infertility. Fertil Steril 1998;70:207-13.
  • 21. Ombelet W, Puttemans P, Bosmans E. Intrauterine insemination: a first-step prosedure in the algorithm of male subfertility treatment. Hum Reprod 1995;10:90- 102.
Annals of Medical Research-Cover
  • Yayın Aralığı: 12
  • Yayıncı: İnönü Üniversitesi Tıp Fakültesi
Sayıdaki Diğer Makaleler

The comparison of vacuum assisted closure (VAC) and conventional treatment methods in postpneumonectomy empyema cases

Özgür Ömer YILDIZ, Göktan TEMİZ, Nurettin KARAOĞLANOĞLU, Ömer Cenap GÜLYÜZ, İlknur Aytekin ÇELİK

Risk factors for pelvic and para-aortic lymph node metastasis in endometrial cancer: A retrospective analysis of 284 patients

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Predisposing factors for failure of conservative treatment in umbilical pilonidal sinus: A clinical experience

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Sex and side differences of three-dimensional Glenoid anthropometric parameters in a normal Turkish population

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Evaluation of physiologic pineal gland calcification via computed tomography in the pediatric population

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Evaluation of the effect of apixaban on the primary intact intervertebral disc cell cultures

Necati KAPLAN, Numan KARAASLAN, İbrahim YILMAZ, Özkan ATEŞ, Feride SİNEM AKGÜN, Duygu Yaşar ŞİRİN, Abdullah Talha ŞİMŞEK, Hanefi ÖZBEK

Risk factors for axillary lymph node metastasis in breast cancer

Uzer KÜÇÜKTULU, Eray KURNAZ, Huseyin EKEN, Bilgin ÇELEBİ

Lactose intolerance in the differential diagnosis of uneasy infants

Münevver Tuğba TEMEL, Levent TEMEL, Mehmet Enes COŞKUN, Abdullah Tuncay DEMİRYÜREK

Effect of diabetes mellitus on prognosis and development of hepatocellular carcinoma in patients with hepatitis C-related cirrhosis

Sezgin VATANSEVER, Halil TAŞKAYNATAN, Gökmen AKTAŞ

Hemolytic disease of the newborn due to minor blood group incompatibility: Sibling cases

Nükhet Aladağ ÇİFTDEMİR, Betül ACUNAŞ, Rıdvan DURAN, Mustafa Törehan ASLAN, Ayşe Ümit TANDIRCIOĞLU, Ülfet Vatansever ÖZBEK