Comparison of the effects of variables in sperm preparation techniques on pregnancy rates in intrauterine insemination
Comparison of the effects of variables in sperm preparation techniques on pregnancy rates in intrauterine insemination
Aim: To investigate the effects of two sperm preparation techniques, density gradient centrifugation (DGC) and swim-up, on thepregnancy rates per cycle and per group in two intrauterine (IUI) cycles.Material and Methods: The retrospective study reviewed 634 patients who presented to Firat University Medical School Hospital andunderwent a total of 1,032 IUI cycles. Group I consisted of 306 (48.3%) patients who underwent a total of 524 IUI cycles betweenJanuary 2012 and January 2016 and Group II included 328 (51.7%) patients who underwent a total of 508 IUI cycles between April 2015and January 2019. The study investigated the effects of technical changes applied to sperm preparation techniques on pregnancyrates in both groups.Results:A significant difference was found between the clinical pregnancy rates of the two groups with regard to the numbers ofpatients and IUI cycles (p
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- 1. Fritz AM, Speroff L. Assisted reproductive technologies. in: clinical gynecologic endocrinology and infertility. 8thth edition. Philadeıphia,: Lippincott Williams 2011;1331-83.
- 2. Tarlatzis BC, Bontis J, Kolibianakis EM, et al. Mantalenakis S.evaluation of intrauterine insemination with washed spermatozoa from the husband in the treatment of infertility. Hum Reprod 1991;6: 1241-46.
- 3. Tomlinson MJ, Amissah-Arthur JB, Thompson KA, et al. Prognostic indicators for intrauterine insemination (IUI): statistical model for IUI success. Hum Reprod 1996;11:1892-1896.
- 4. Rappa KL, Rodriguez HF, Hakkarainen GC, et al. Sperm processing for advanced reproductive technologies: Where are we today? Biotechnol Adv 2016;34:578-87.
- 5. Paasch U, Grunewald S, Glander HJ. Sperm selection in assisted reproductive techniques. Soc Reprod Fertil Suppl 2007;65:515-25.
- 6. Özkavukçu S, Aras D. Sperm Preparation Methods for IUI and IVF. TJRMS 2017;1:92-8.
- 7. Mortimer D. Sperm recovery techniques to maximize fertilizing capacity. Reprod Fertil Def 1994;6:25-31.
- 8. Agarwal A, Nallella KP, Allamaneni SS, et al. Role of antioxidants in treatment of male infertility: an overview of the literatüre. Reprod Biomed 2004;8:616- 27.
- 9. O’Flaherty C, Lamirande E, Gagnon C. Positive role of reactive oxygen species in mammalian sperm capacitation: triggering and modulation of phosphorylation events. Free Radic Biol Med 2006;41:528–40.
- 10. Said TM, Agarwal A, Sharma RK. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 2003;79:829–43.
- 11. Benchaib M, Braun V, Lornage J, et al.Guerin JF. Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique. Hum Reprod 2003;18:1023-8.
- 12. Henkel R, Kierspel E, Stalf T, et al. Schill WB, kruger TF. effect of reactive oxygen species produced by spermatozoa and leucocytes on sperm functions in non-leukocytospermic patients. Fertil Steril 2005;83:635-42.
- 13. Henkel RR, Schill WB. Sperm preparation for ART. Reprod Biol Endocrinol 2003;1:108-29.
- 14. Enginsu ME, Pieters MH, Dumolin JC, et al. Male factor as determinant of in vitro fertilization outcome. Hum Reprod 1992;7:1136-40.
- 15. Amiri I, Ghorbani M, Heshmati S. Comparison of the DNA fragmentation and the Sperm parameters after processing by the density gradient and the swim-up methods. J Clin Diagn Res 2012;6:1451-3.
- 16. Tomlinson MJ, Moffatt O, Manicardi GC, , et al. Interrelationships between seminal parameters and sperm nuclear DNA damage before and after density gradient centrifugation implications for assisted conception. Hum Reprod 2001;16:2160-5.
- 17. Agarwal A, Said TM. Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum Repro 2003;9:331-45.
- 18. Aitken RJ, De Iuliis GN. Origins and consequences of DNA damage in male germ cells. Reprod Biomed 2007;14:727-33.
- 19. Bungum M, Humaidan P, Axmon A, et al.Giwercman A. sperm DNA integrity assessment in prediction of assisted reproduction technology outcome. Hum Reprod 2007;22:174-9.
- 20. Guzick D, Sullivan M, Adamson GD, et al. Steinkampf MP. Efficacy of treatment for unexplained infertility. Fertil Steril 1998;70:207-13.
- 21. Ombelet W, Puttemans P, Bosmans E. Intrauterine insemination: a first-step prosedure in the algorithm of male subfertility treatment. Hum Reprod 1995;10:90- 102.