GENİTOÜRİNER SİSTEM ÖRNEKLERİNDE ÜREYEN UREAPLASMA UREALYTICUM VE MYCOPLASMA HOMINIS KÖKENLERİNİN İN VİTRO ANTİBİYOTİKLERE DUYARLILIKLARININ DEĞERLENDİRİLMESİ

Genitoüriner sistem (GÜS) infeksiyon etkenlerinden olan Ureaplasma urealyticum ve Mycoplasma hominis prevalansı sosyoekonomik durumla ilişkilidir. Bu mikroorganizmaların antibiyotik duyarlılık profilleri bölgesel değişiklik göstermektedir. Çalışmamızda GÜS infeksiyonu şüphesi ile laboratuvarımıza gönderilmiş olan çeşitli GÜS örneklerinde U.urealyticum ve M.hominis sıklığının ve antibiyotik duyarlılık oranlarının belirlenmesi amaçlanmıştır.U.urealyticum ve/veya M.hominis infeksiyonu şüphesi ile laboratuvarımıza gönderilmiş olan 3410 hastanın idrar, vajinal akıntı, servikal sürüntü, semen ve üretral akıntıdan oluşan GÜS örnekleri, retrospektif olarak değerlendirilmiştir. U.urealyticum ve/veya M.hominis üremesinin tanımlanmasında ve antibiyotik duyarlılık testlerinde Mycoplasma IES (AutobioDiagnostics, Çin) kiti kullanılmıştır. Sonuçlar CLSI kriterlerine göre duyarlı, orta duyarlı ve dirençli olarak kategorize edilmiştir.Değerlendirilen örneklerin % 22.2'sinde U.urealyticum, % 2.9'unda U.urealyticum ve M.hominis koenfeksiyonu ve % 0.2'sinde M.hominis üremesi saptanmıştır. Duyarlılık oranlarının en yüksek olduğu antibiyotikler, U.urealyticum için minosiklin (% 98.5), doksisiklin (% 98.1) ve josamisin (% 98.1); M.hominis için doksisiklin (% 96.2), minosiklin (% 94.3) ve josamisin (% 88.7) olarak belirlenmiştir. Verilerimiz incelenen hasta grubunda U.urealyticum'un M.hominis'e göre belirgin olarak daha sık saptandığına, üremelerin 20-29 yaş aralığında ve kadınlarda daha sık (p=0.034) görüldüğüne, minosiklin ve doksisiklin duyarlılık oranlarının her iki bakteri için de oldukça yüksek olduğuna işaret etmektedir

Evaluation of In Vitro Antimicrobial Susceptibility in Ureaplasma urealyticum and Mycoplasma hominis Strains Isolated from Genitourinary Tract Samples

Prevalence of genitourinary tract infections caused by Ureaplasma urealyticum and Mycoplasma hominis are related to sociodemographic status. Antimicrobial susceptibility patterns of these microorganisms vary regionally. In our study, it is aimed to determine frequency of U.urealyticum and M.hominis in genitourinary tract samples collected from patients with suspicion of genitourinary tract infection and, their antimicrobial susceptibility ratios. Genitourinary tract samples consisting of urine, vaginal discharge, cervical swab, semen and urethral discharge obtained from 3410 patients suspected with genitourinary infection caused by U.urealyticum and/or M.hominis were evaluated retrospectively. Mycoplasma IES commercial kit (AutobioDiagnostics, China) was used for identification and, antimicrobial susceptibility testing of U.urealyticum and/or M.hominis. Susceptibility test results were categorized as susceptible, intermediate or resistant according to CLSI criteria. While U.urealyticum, and M.hominis was detected from 22.2 % and 0.2 % of the samples respectively; co-infection was detected in 2.9 %. The most effective antimicrobials against both U.urealyticum and M.hominis were minocycline (98.5 % and 94.3 %), doxycycline (98.1 % and 96.2 %) and josamycin (98.1 % and 88.7 %). Our results indicate that U.urealyticum was clearly more frequent than M.hominis, positive culture results were more common in 20-29 years and females (p=0.034), and minocycline and doxycycline susceptibility ratios were considerably high against both microorganisms.

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