Can Kezban ŞAHNA, Feray ALKAN, İbrahim BURGU, Can ÇOKÇALIŞKAN

Yeni doğan buzağı ishallerine karşı ticari aşı ile aşılanan sığırlardan doğan yavrularda pasif bağışıklık düzeyi

Bu çalışmada, bir ticari aşı (ScourGuard®(K),Pfizer) ile aşılanan sığırlarda BRV ve BCV spesifik bağışıklığın düzeyi ile yeni doğanların enfeksiyonunda pasif bağışıklığın rolü incelendi. Bu amaçla gebeliğinin 3. trimesterinde bulunan 41 sığırdan 2 grup oluşturuldu. Grup I'de bulunan sığırlara ticari aşı prospektüsüne göre aşı uygulandı. Grup II ise kontrol grubu olarak değerlendirildi. Her iki gruptaki sığırlardan aşı uygulamalarına paralel, doğum yaptıkları gün (l.gün) ve sonraki 3, 7, 14, 21 ve 28. günlerde kan, gaita ve kolostrum/süt (aşı uygulama dönemleri hariç) örnekleri; yeni doğan buzağılardan doğum sonrası 3, 7, 14, 21, 28. günlerde kan ve gaita örnekleri alındı. Kan ve kolostrum/süt örnekleri BRV ve BCV spesifik antikorlar yönünden mikronötralizasyon, gaita örnekleri BRV ve BCV antijeni yönünden ELISA ile kontrol edildi. Elde edilen verilere göre, aşı uygulanan ve aşı uygulanmayan sığırlara ait kan serumu ve kolostrum/süt örneklerindeki antikor seviyeleri ile yeni doğan buzağıların pasif bağışıklık düzeyi ve enfeksiyona maruz kalma oranlan karşılaştırmalı olarak değerlendirildi. Aşı uygulamasını takiben erişkin sığırlarda aşı etkenlerine spesifik antikor yanıtında belirgin bir artış saptanamadı. Ancak aşı uygulanan ve aşı uygulanmayan annelerden doğan buzağılarda enfeksiyona maruz kalma oranlarının sırasıyla %30.0 ve %54.5 olduğu belirlendi.

Level of the passive immunity in calves from cows vaccinated for neonatal calf diarrhea

In this study, the level of the BRV and BCV specific immunity in cows which vaccinated with a commercial vaccine (ScourGuard®(K),Pfizer) and the role of the passive immunity in newborns' infections were investigated. For this aim two groups were organised from 41 cows in their 3rd trimaster of pregnancy. Group I cows were vaccinated according to the prosedüre of the commercial vaccine. Group II cows were used as a control . At the time of the vaccination, at the calving day (lsl day) and at 3,7,14,21 and 28th days after calving, blood, feces and colostrum/milk (except vaccination period) samples from cows in each group; from newborn calves at 3,7,14,21,28th days after birth blood and feces samples were collected. Blood and colostrum/milk samples were controlled for the presence of BRV and BCV antibodies using microneutralisation technique. Feces were tested for BRV and BCV antigen by ELISA. The antibody liters in blood sera and colostrum/milk from cows, levels of the passive immunity of newborn calves and the infection rates of calves were evaluated comparatively for Group I and II cows, their calves. Data shown that following the vaccination no remarkable specific antibody increase against vaccine antigens (BRV and BCV) was detected in cows. However the infection rate of calves born from vaccinated and unvaccinated cows were 30.0% and 54.5%, respectively.

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