Bayazıt MUSAL, B. Hakkı BECERİKLİSOY, A. Pınar. ULUTAŞ

The effect of preoperative antibiotic or antibiotic + vitamin C administrations on the inflammatory and oxidative state in the rabbits with experimentally induced pyometra

Bu çalışmada pyometra operasyonu sonrası stresin azaltılmasında preoperatif sağaltımın etkisini araştırmak amacıyla tavşanlar model olarak kullanıldı. Bu amaçla onsekiz sağlıklı tavşanda Pasteurella multocida nın operatif yöntemle uterusa inokulasyonu gerçekleştirildi. İnokulasyondan sonra 3. günden başlanarak Grup I e (n=6) enrofloksasin (2,5 mg/kg, s.c.), Grup II ye (n=6) aynı dozda enrofloksasin ve vitamin C (100 mg, s.c.) enjeksiyonları 3 gün süreyle devam ettirilmiş ve bundan sonra ovaryohisterektomi uygulanmıştır. Kontrol grubu olan Grup III e (n=6) medikal sağaltım uygulanmamış ve inokulasyondan sonra üçüncü günde ovaryohisterektomi uygulanmıştır. Kan örnekleri inokulasyondan önce, ovaryohisterektomi gününde, postoperatif 24. saat, 72. saat ve 7. günde alınmıştır. Bu örneklerde serüloplazmin (CERU), malondialdehit (MDA), vitamin C, haptoglobin (Hp) ve Serum Amiloid A (SAA) düzeyleri belirlenmiştir. Serüloplazmin bütün gruplarda inokulasyonu takiben yükselirken Grup I ve II de postoperatif 72s de düşmüş ancak Grup III de yüksek bulunmuştur (p

Tavşanlarda deneysel oluşturulan pyometra olgularında, preoperatif antibiyotik veya antibiyotik + C vitamini enjeksiyonlarının yangısal ve oksidatif duruma olan etkileri

In this study, rabbits were used as a model for canine pyometra to test the effects of preoperative therapy in the reduction of postoperative stress. Pyometra were induced in 18 healthy rabbits by the operative administration of Pasteurella multocida inoculum. Beginning on the third day after inoculation Group I (n=6) received enrofloxacin (2,5 mg/kg, s.c.), Group II (n=6) recieved enrofloxacin (2,5 mg/kg, s.c.) + vitamin C (100 mg, s.c.) injections for three days. Ovariohysterectomy were performed in groups I and II following this medical treatment. Group III (n=6) as the control group, did not recieve any medical therapy and were operated on the third day after inoculation. Blood samples were collected before inoculation, on the day of operation, 24th h, 72th h and at 7th day following the operations. Ceruloplasmin (CERU), malondialdehyde (MDA), vitamin C, haptoglobin (Hp) and serum amyloid-A (SAA) levels were analyzed. In the postoperative 72th h period mean CERU level of Group III were found significantly higher (p

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