Suleyman YAZAR, Izzet SAHIN, Salih KUK, Ulfet CETINKAYA, Yunus UYAR

Leishmania sp. in Visceral Leishmaniasis suspected patients in Kayseri

Dumdum ateşi veya kala-azar olarak bilinen, aralıklı ateş, kilo kaybı, hepatosplenomegali ve pansitopeni ile karakterize visseral leishmaniasis (VL), tedavi edilmezse iki yılda %100 ölüm oranına sahip olabilmektedir. Bu çalışmada, Ocak 1995 ve Ağustos 2012 arasında Erciyes Üniversitesi Tıp Fakültesi Parazitoloji Anabilim Dalı'na VL şüphesi ile başvuran hastalarda Leishmania sp. araştırılması amaçlanmıştır. Ocak 1995 ile Ocak 2004 tarihleri arasında 37 (%54.4)si erkek ve 31 (%45.6)i kadın olmak üzere toplam 68 VL şüpheli hastadan alınan kemik iliği örneği Giemsa boyama tekniği ile incelenmiştir. Mikroskobik inceleme sonucunda 4 (%5.9) hastada Leishmania amastigotları tespit edilmiştir. Ocak 2004 ve Ağustos 2012 tarihleri arasında 42 (%55.3)'si erkek ve 34 (%44.7)'ü kadın olmak üzere toplam 76 VL şüpheli hastadan alınan kemik iliği ve serum örnekleri Giemsa boyama ve İndirekt Fluoresan Antikor (IFA) teknikleri ile incelenmiştir. İncelenen hastalardan 3 (%3.9)'ü hem IFA hem de Giemsa boyama tekniği ile pozitif bulunmuştur. Dört (%5.3) hasta ise yalnızca IFAT ile pozitif bulunmuştur. Sonuç olarak VL Kayseride bir sağlık sorunudur ve VL'ye karşı mücadele özel programlar ile desteklenmelidir. Bununla birlikte, VL tanısında IFA ve Giemsa boyamanın birlikte kullanılması daha güvenli olacaktır.

Kayseri'de Visseral Leishmaniasis şüpheli hastalarda Leishmania sp.

Visceral leishmaniasis (VL), also known as dumdum fever or kala-azar, is characterized by intermittent fever, substantial weight loss, hepatosplenomegaly and pancytopenia. If left untreated, the disease can have a fatality rate as high as 100% within two years. This study was aimed to investigate the presence of Leishmania sp. in VL suspected patients who applied to Erciyes University Medical Faculty Department of Parasitology between January 1995 and August 2012. Bone marrow samples obtained from 37 (54.4%) males and 31 (45.6%) females making a total of 68 VL suspected patients between January 1995 and January 2004 were examined by Giemsa stain technique. Microscopic examination showed Leishmania amastigotes in 4 (5.9%) patients. Bone marrow and sera samples obtained from 42 (55.3%) males and 34 (44.7%) females making a total of 76 VL suspected patients between January 2004 and August 2012 were examined by Giemsa stain technique and Indirect Fluorescent Antibody Test (IFAT). Three (3.9%) patients were found to be positive with both IFAT and Giemsa stain technique. Four (5.3%) patients were found to be positive with only IFAT. As a result, VL is a health problem in Kayseri and the fight against VL must be supported by special programs. Therefore, we believe that it should be safer to use both Giemsa stain technique and IFAT together for diagnosis of VL.

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