Gülnur SERDAR, Özlem BÜYÜKTANIR YAŞ, Evrim GENÇ, Oktay GENÇ

Sığırlarda Brucella abortus S19 konjunktival aşısına karşı immün yanıtın izlenmesi

Brucella abortus S19 suşu sığırlarda brusellozise karşı en çok tercih edilen aşı suşlarından biridir. Bununla birlikte, B. abortus S19 aşısına bağlı immün yanıtın izlenmesi immünoreaktif bir antijeninin ve test yönteminin belirlenememesi nedeniyle zordur. Bu çalışma ile düve ve buzağılarda bu aşıya karşı gelişen humoral ve hücresel immün yanıtın aşılama sonrası örneklemenin yapıldığı 46, 85 ve 169. günlerde izlenmesi amaçlandı. Bu nedenle, humoral immünite için, lipopolisakkarit (LPS) antijenine karşı immünoglobulin izotip IgG ve IgA'nın ve hücresel immün yanıt için ise brusellerjen antijenine karşı hücresel immünyanıt göstergelerinden interferon gamma (IFNg) home-made ELISA yöntemleri ile araştırıldı. Bu çalışmada, aşılama sonrası 46. günde buzağılarda (%100) ve düvelerde (%96.6) belirgin IgG pozitifliği gözlendi, fakat IgA pozitifliği ve IFNg düzeyleri %50’nin üzerine çıkmadı. Ayrıca, IFNg tespiti için %13.3-43.3 ve IgA tespiti için %0-44 aralığında belirlenen pozitif hayvan yüzde oranları aşı takibi için yetersiz olarak belirlendi. Bu nedenle, sığırlarda B. abortus S19 konjunktival aşısının etkinlik takibinde IgG düzeyleri kullanılabilir. Diğer taraftan, immüniteyi izlemek için brusellerjen ve LPS ile birlikte yeni antijen kombinasyonlarının kullanılması durumunda testlerin duyarlılığı artacak olup, gelecekteki araştırmalar için önerilmektedir.

Monitoring of the immune response to B. abortus S19 conjunctival vaccine in cattle

Brucella abortus S19strain is one of the most preferred strains in vaccines against brucellosis in cattle. However, monitoring of the B. abortus S19 vaccine is difficult due to non availability of sustainable immunoreactive antigen and accurate test method. In this study, the humoral and the cellular immune responsesto S19 vaccine in one year old heifers and calves were monitored on post vaccination days (pvd) of 46, 85 and 169. Thus, the levels of Immunoglobulin (Ig)G and IgA isotypes against lipopolysaccharide (LPS) for humoral immunity and interferon gamma (IFNg) against brucellergen for cellular immune response were investigated by home-made ELISAs. In this study, significant IgG positivity was observed on pvd 46 in calves (100%) and heifers (96.6%), but IgA positivity and IFNg levels were not over 50%. Moreover, percentage of positive animals for IFNg (13.3-43.3%) and IgA (0-44%) have shown no significance for monitoring the vaccine throughout the study. Therefore, IgG levels can be used to monitor the efficiency of Brucella abortus S19 conjunctival vaccine in cattle. On the other hand, novel antigen combinations along with brucellergen and LPS for monitoring the immunity would enhance the sensitivity of the test and could be recommended for future investigations.

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