Haşmet ÇAĞIRGAN, Ebru YILDIRIM, Emine BAYDAN, Sezai KAYA, Levent ALTINTAŞ, Begüm YURDAKÖK, Farah Gönül AYDIN, Aslı Gül KÜÇÜKOSMANOĞLU, Hüsamettin EKİCİ

Investigation of some veterinary drug residues in sea water, sediment, and wild fishes captured around fish farms in the Aegean Sea: Oxytetracyline, ivermectin and emamectin

Besinlerdeki ilaç ve metabolitlerinin kalıntıları ilgili yerel makamlar tarafından düzenli olarak araştırılmaktadır; ancakbu kalıntıların çevredeki akıbeti bilinmemektedir. Akvakültür endüstrisinin giderek artan önemi ve antibiyotiklerin bu endüstri ilebirlikte kullanımının giderek yaygınlaşmasına rağmen doğal balık, sediment ve deniz suyu kalıntıları ile ilgili araştırmala r Türkiyedesınırlıdır. Bu araştırmada oksitetrasiklin, ivermektin ve emamektin benzoat kalıntıları; ilaç kullanımının daha fazla olduğu dört ayiçerisinde, Ege Denizi, Bodrum, Salihli Adası çevresindeki balık çiftlikleri etrafından avlanan doğal ortam ba lıkları (Obladamelanura, Mullus barbatus ), deniz suyu ve sediment örneklerinde Yüksek Basınçlı Sıvı Kromatografisi (YBSK) yöntemikullanılarak ve her bir matriksin validasyonunu takiben araştırıldı. Numunelerde, valide edilmiş metodlardaki tespit limiti ü zerindeherhangi bir kalıntıya rastlanmadı. Akvakültürde kullanılan veteriner antibiyotiklerinin ekosistem üzerinde özellikle düşük dozlardaakümülasyona bağlı oluşabilecek potansiyel risklerinin değerlendirilebilmesi için, farklı analitlerde tarama testle rininyaygınlaştırılması ve arttırılması yararlı olacaktır.

Ege Denizinde balık çiftlikleri etrafında avlanan doğal ortam balıkları ile çevredeki su ve sedimentin bazı veteriner ilaç kalıntıları yönünden incelenmesi: Oksitetrasiklin, ivermektin ve emamektin

Veterinary drug residues and their metabolites in food are regularly investigated by local authorities; howevertheir fate in the environment is still unknown. Despite the importance of the aquaculture industry and the widespread use ofantibiotics; limited scientific information regarding their residue in natural fish, sediment a nd sea water are available in TURKEY.The current study; which is the first study in this area, was undertaken to determine the oxytetracycline (OTC), ivermectin ( IVM) andemamectin benzoate (EMA) residues from samples of wild fishes ( Oblada melanura, Mullus barbatus), sea water and sedimentcollected in four different months, caught around the fish cages near Salihli Island in Bodrum, Aegean Sea. Samples were analyzedby High Pressure Liquid Chromatography (HPLC) followed by the validation for each matrix. No residues were found to be abovethe Limit of detection (LOD) levels of the validated methods in the screened samples. In order to understand the possible risk ofveterinary antibiotics, especially for low dose accumulation, to the ecosystem for sustain able aquaculture, conduction of morescreening analysis with expanded possible matrices would be beneficial.

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