Hasan ALKAN, Kübra KARAKAŞ, Ayhan BAŞTAN, Tevhide SEL, Halit KANCA, Seçkin SALAR, İdil BAŞTAN

Serum ceruloplasmin and paraoxonase- 1 levels in ovariectomized and ovariohysterectomized dogs

Bu çalışmanın amaçları köpeklerde ovariektomi (OVE) ve ovaryohisterektomi (OVH) ile ilişkili akut faz yanıtınınşiddetinin değerlendirilmesi ve paraoksanaz -1 (PON -1) aktivitesinin araştırılmasıydı. Çalışmada toplam 21 adet sağlıklı dişi köpekkullanıldı. Köpekler rastgele OVH (n=11) ve OVE (n=10) yapılmak üzere iki gruba ayrıldı. Operasyon öncesinde ve operasyonsonrası 4. günde alınan periferal kan örneklerinde serum seruloplazmin düzeyi ve paraoksonaz -1 aktivitesi ölçüldü. Sonuçlar gruplariçerisinde ve gruplar arasında karşılaştırıldı. Paraoksanaz -1 ve seruloplazmin arasında muhtemel korelasyon değerlendirildi.Operasyon öncesi serum seruloplazmin seviyeleri O VE ve OVH gruplarında sırasıyla 0.80±0.17 and 1.65±0.35 U/ml olarak ölçüldü(P>0.05). Operasyon sonrası 4. günde serum seruloplazmin seviyesi OVH grubunda (1.95±0.47 U/ml) OVE grubuna kıyasla dahayüksek bulundu (0.69 ±0.10 U/ml; P0.05). Operasyon öncesi ve sonrasında serum PON-1 aktivitesi OVE ve OVH gruplarında sırasıyla1001.56±44.60 U/ml ve 1119.10±87.99 U/ml olup (P>0.05), operasyon sonra sı serum PON-1 aktivitesi OVH grubunda(1065.15±46.00 U/ml) OVE grubuna kıyasla daha yüksekti (941.22±32.57 U/ml; P

Ovariektomi ve ovaryohisterektomi yapılan köpeklerde serum seruloplazmin ve paraoksonaz- 1 düzeyleri

The objectives of this study were to evaluate the severity of acute phase reaction and to investigate theparaoxanase -1 (PON -1) activity associated with ovariectomy (OVE) and ovariohysterectomy (OVH) in dogs. A total of 21 adulthealthy dogs were included. The dogs were randomly divided into two groups and either ovariectomized (n=10) orovariohysterectom ized (n=11). Peripheral blood samples were collected preoperatively and on postoperative fourth day for serumceruloplasmin (cp) and paraoxanase -1 activity measurements. Results were compared within and between groups. Potentialcorrelation between paraoxanase -1 and ceruloplasmin was assessed. Preoperative mean serum ceruloplasmin levels in OVE andOVH groups were 0.80±0.17 and 1.65±0.35 U/ml, respectively (P>0.05). On postoperative 4th day, serum ceruloplasmin levels inOVH group (1.95±0.47 U/ml) was higher compared to OVE group (0.69±0.10 U/ml; P0.05). Preoperative serum paraoxanase-1 activity in OVE and OVHgroups were 1001.56±44.60 U/ml and 1119.10±87 .99 U/ml, respectively (P>0.05). Higher postoperative serum paraoxanase-1activity was observed in OVH group (1065.15±46.00 U/ml) compared to OVE group (941.22±32.57 U/ml; P0.05). Paraoxanase-1 activity was not correlatedwith concentration of ceruloplasmin (P=.250; r=0.06). In conclusion, OVE is superior to OVH in terms of reducing the severity ofpostoperative acute phase response. In addition, OVE and OVH does not alter canine paraoxanase-1 activity on postoperative fourthday and paraoxanase-1 activity is not associated with serum concentration of ceruloplasmin in dogs.

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1. Ceron JJ, Eckersall PD, Subiela SM (2005): Acute phase proteins in dogs and cats: current knowledge and future perspectives. Vet Clin Pathol, 34, 85 -99.
2. Ceron JJ, Subiela SM (2004): An automated spectrophotometric method for measuring canine ceruloplasmin in serum. Vet Res, 35, 671 -679.
3. Conner JG, Eckersall PD, Ferguson J, Douglas TA (1988): Acute phase response in the dog following surgical trauma. Res Vet Sci, 45, 107-110.
4. De Tora M, McCarty RJ (2011): Ovariohysterectomy versus ovariectomy for elective sterilization of female dogs and cats: is removal of the uterus necessarry? . JAVMA, 239, 1409-1412.
5. Eckersall PD (2008): Proteins, Proteomics and Dysproteinemias. 117-155. In: JJ Kaneko, JW Harvey, ML Bruss, Clinical Biochemistry of Domestic Animals. 6th Ed, Academic Press, San Diego.
6. Goethem BV, Schaefers-Okkens A, Kirpensteijn J (2006): Making a rational choice between ovariectomy and ovariohysterectomy in the dog: a discussion of the benefits of either technique. Veterinary Surgery, 35, 136- 143.
7. Gunay A, Gunes N, Gunay U (2011): Effect of ovariohysterectomy on lipid peroxidation and levels of some antioxidants and biochemical parameters in bitches. Bull Vet Inst Pulawy, 55, 695 -698.
8. Kirpensteijn J (2008): Ovariectomy versus ovariohysterectomy. Is the eternal argument ended. Proceedings of the international SCIVAC Congress, Rimini, Italy, 290-293.
9. Kum C, Voyvoda H, Sekkin S, Karademir U, Tarimcilar T (2013): Effects of carprofen and me loxicam on C-reactive protein, ceruloplasmin, and fibrinogen concentrations in dogs undergoing ovariohysterectomy. Am J Vet Res, 74, 1267-1273.
10. Lee JY, Kim MC (2014): Comparison of oxidative stress status in dogs undergoing laparoscopic and open ovariectom y. J Vet Med Sci, 76, 273 -276.
11. Rossi G, Giordano A, Pezzia F, Kjelgaard-Hansen M, Paltrinieri S (2013): Serum paraoxonase 1 activity in dogs: preanalytical and analytical factors and correlation with C-reactive protein and alpha-2-globulin. Vet Clin Pathol, 42, 329-341.
12. Serin G, Kiral F, Serin I (2008): Acute effect of ovariohysterectomy on lipid peroxidation and some antioxidant levels in dogs. Bull Vet Inst Pulawy, 52, 251 - 253.
13. Sunderman FW, Nomoto S (1970): Measurement of human serum ceruloplasmin by its p- phenylenediamine oxidase activity. Clin Chem, 16, 903 -910.