Ongun UYSAL, Serpil SARIÖZKAN, Ergun AKÇAY, Necmettin TEKİN

Effects of different serum types in in vitro culture of in vitro fertilized rabbit oocytes

Bu çalışmada 33 Yeni Zelanda Beyaz dişi ve 2 erkek tavşan kullanıldı. HCG enjeksiyonu ile ovulasyonun uyarılmasının ardından ovidukt yıkanarak oositler toplandı. Oosit ve spermatozoa fertilizasyon için mDM’da 5 saat inkübe edildi. Kültürde serumun etkisinin araştırılması amacıyla % 20 FCS ve % 20 Tavşan Serumu katılmış Ham’s F-10 mediumunda 18-21 saat kültüre edildi. Fertilizasyon oositlerin bölünme oranları saptanarak tespit edildi. Fertilizasyon oranları FCS veya RS veya hiç serum ilave edilmemiş kültür mediumunda inkübe edilen oositlerde sırasıyla % 70.4, % 73.8, % 60.6 olarak kaydedildi (p>0.05). Verilerin değerlendirilmesinde ki-kare testi kullanıldı. Tavşan serumu ilave edilmiş olan Ham’s F-10 mediumunda fertilizasyon oranının daha yüksek olmasına karşın grup ortalamaları arasında istatistiki açıdan fark yoktur.

In vitro fertilize tavşan oositlerinin in vitro kültüründe farklı serum tiplerinin etkileri

In this study 33 New Zealand White does and 2 bucks were used. After induction of ovulation by human chorionic gonadotrophin (hCG) injection, oocytes were collected by flushing of oviduct. Oocytes and spermatozoa were incubated for 5 hours in Modifie Defined Medium (mDM) for fertilization and cultured in Ham’s F-10 medium supplemented with 20 % Fetal Calf Serum (FCS) and 20 % Rabbit Serum (RS) for 18-21 hours, to evaluate the effect of serum in fertilization. Fertilization was assessed by detection of oocytes cleavage rates. The fertilization rates were recorded as 70.4 %, 73.8 %, 60.6 % in oocytes incubated in culture mediums with FCS or RS or without any serum respectively (p>0.05). Data were evaluated via Chi-square test. These data suggest that the differences between group averages were not statistically significant, although the fertilization rate in Ham’s F-10 with rabbit serum was higher.

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