Ahmet ATA, Latife ÇAKIR BAYRAM²

Deneysel olarak Arcobacter butzleri ile infekte edilmiş Balb/c farelerde bakteriyel antijenin immunohistokimyasal dağılımı ve histopatolojik bulgular

Bu çalışmada BALB/c farelerde Arcobacter butzleri'nin (A.butzleri) patojenitesini ve doku kolonizasyonunu ortaya koymak amaçlanmıştır. Toplam 60 adet fare kullanıldı. Her birinin deney grubunda 16 adet, kontrollerinde ise 4'er adet fare kullanılan 3 grup oluşturuldu. Araştırma 15 günle sınırlandırıldı. A.butzleri izolatı ml'sinde 1x109 bakteri içerecek şekilde BALB/c farelere peroral (p.o), intraperitoneal (i.p) ve meme bezi içine (m.b.i) verilerek klinik ve patolojik bulgular değerlendirildi. BALB/c fareler üç inokulasyon yoluyla da infeksiyona duyarlı bulundular. Mortalite, i.p ve m.b.i inokulasyon gruplarında %95, p.o grupta ise %25 olarak saptandı. Klinik ve patolojik bulgular i.p. ve m.b.i. inokulasyon gruplarında gözlendi. İnfekte gruplarda, ishal, halsizlik, vücut ısısının düşmesi (i.p ve m.b.i); hepatomegali, splenomegali (i.p); infekte meme bezlerinde şişkinlik, eksudasyon, apse formasyonu (m.b.i) gözlendi. Histopatolojik olarak, akciğer, karaciğer, böbrek, dalak, bölgesel lenf düğümleri, mide, kolon ve nötrofil ve plazma hücreleri ile karakterize akut/kronik yangı, lokal nekroz ve konjesyon (i.p), meme bezi ve deri altı dokusunda ise şiddetli, diffuz, kronik pyogranulomatoz-nekrotik yangı ve intersitisyel fibrosis (m.b.i) saptandı. Tüm inokulasyon gruplarında Arcobacter butzleri inokülasyon sonrası 5. güne kadar izole edildi. Sonuç olarak, BALB/c farelerin Arcobacter butzleri'nin deneysel infeksiyonu için uygun deney hayvanı olarak kullanılabileceği kanısına varıldı.

Histopathological findings and immunohistochemical distribution of bacterial antigen in Balb/c mice experimentally infected with Arcobacter butzleri

The aim of this study was to determine the tissue colonization and pathogenicity of Arcobacter butzleri in BALB/c mice. For this purpose, 60 adult mice were distributed into three experimental groups consisting of 16 animals in each and into three control groups consisting of 4 animals in each. One ml of A. butzleri suspension containing 1x109 CFU/mL was inoculated by peroral (PO), intraperitoneal (IP) and intramammary (IMG) routes to BALB/c mice in experimental groups in order to determine the clinical and pathological findings. Animals in control groups received 1 mL of sterile saline solution. Duration of the experiment was limited for 15 days. The BALB/c mice were found to be susceptible to infection by the three ways of inoculation. In infected mice, diarrhea, weakness, decrease of body temperature (IP and IMG); hepatomegaly, splenomegaly (IP); swelling of mammary glands, exudation, abscess formation (IMG) were seen. Mortality was determined as 25% in PO inoculation group and as 95% in IP IMG inoculation groups. In histopathological examination, acute/chronic inflammation characterized with neutrophil and plasma cells and local necrosis or congestion (IP) were determined in the lungs, kidneys, regional lymph nodes, liver, spleen, stomach and colon. However, severe, diffuse and chronic pyogranulomatous and necrotizing inflammation and interstitial fibrosis were observed in mammary gland and its subcutaneous tissues (IMG). In all of inoculation groups, A. butzleri was reisolated until the 5th days of post inoculation. The results of this study have suggested that BALB/c mice might be an appropriate experimental animal model for studying A.butzleri infection.

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