Prostat Karsinomlarında E-kadherin ve Beta-katenin Ekspresyonunun Gleason Skoru ve Diğer Prognostik Faktörler ile Karşılaştırılması

Amaç: Hücre adezyon kompleksi E-kadherin ve beta-kateninin, epitelyal hücrelerin doku bütünlüğü, organizasyonu, epitelyal fenotipinkorunması,transkripsiyon ve proliferasyonda önemli rolleri vardır. Bazı epitelyal malignitelerde bu moleküllerde değişiklikler bildirilmiştir. Buçalışmada prostat karsinomlarında E-kadherin ve beta-kateninin immünhistokimyasal ekspresyon durumunun Gleason skoru ve diğer prognostikfaktörler ile ilişkisi değerlendirilerek prostat karsinomu patogenezinde bu moleküllerin yeri araştırıldı.Gereç ve Yöntem: Çalışmaya 2003-2008 yılları arasında Gazi Üniversitesi Tıp Fakültesi Patoloji Anabilim Dalı’na gelen ve prostat adenokarsinomutanısı alan 156 radikal prostatektomi olgusu dahil edildi. Olguların prognostik faktörleri retrospektif olarak incelenerek kaydedildi. Gleason skorunagöre olgular gruplara ayrıldı. E-kadherin ve beta-katenin antikorları ile tümör hücreleri immünhistokimyasal ekspresyon yüzdeleri ve şiddetine göresemikantitatif olarak değerlendirildi. E-kadherin ve Beta-katenin ekspresyon düzeyleri ile Gleason skoru ve diğer prosnostik faktörler arasındaki ilişkiistatistiksel olarak araştırıldı.Bulgular: E-kadherin ile 156 olgunun 47’sinde (%30) ekspresyon kaybı izlendi. E-kadherin ekspresyon kaybı ile yüksek Gleason skoru ve seminalvezikül invazyonu arasında istatistiksel olarak anlamlı ilişki saptandı. Beta-katenin ile 156 olgunun 79’unda (%50) ekspresyon kaybı saptandı.Beta-katenin ekspresyon kaybı ile yüksek Gleason skoru, seminal vezikül invazyonu ve prostat dışı yayılım arasında istatistiksel olarak anlamlı ilişkisaptandı.Sonuç: Çalışmamızda E-kadherin ve beta-katenin ekspresyon kaybı ile yüksek Gleason skoru, seminal vezikül invazyonu ve prostat dışı yayılımarasında anlamlı ilişki saptadı. Bulgular prostat karsinomu patogenezinde bu moleküllerinin rolünü destekledi. Ancak çalışmada metastatik tümörlerinolmaması, klinik evre ve klinik takip ile ilişkilerinin değerlendirilmemesi nedeni ile agresif seyirli tümörlerde bu moleküllerin rolü hakkında bir yorumyapılamadı.

Comparison of E-cadherin and Beta-catenin Expression with Gleason Score and Other Prognostic Factors in Prostate Carcinomas

Objectives: Cell adhesion complex E-cadherin and beta-catenin have important roles in tissue integrity, organization, protection of epithelial phenotype, transcription and proliferation of epithelial cells. Changes in these molecules have been reported in some epithelial malignancies. In this study, with the evaluation of the role of E-cadherin and beta-catenin immunohistochemical expression status in prostate carcinomas with Gleason score and other prognostic factors and the role of these molecules in the pathogenesis of prostate carcinoma were investigated. Materials and Methods: One hundred fifty-six radical prostatectomy cases diagnosed as prostate adenocarcinoma between 2003 and 2008 years at the Gazi University, Faculty of Medicine, Department of Pathology, were included in the study. The prognostic factors of the cases were retrospectively reviewed and recorded. The cases were divided into groups according to the Gleason score. E-cadherin and beta-catenin antibodies and tumor cells were evaluated semi-quantitatively according to the percentages and severity of immunohistochemical expression. The relationship between E-cadherin and beta-catenin expression levels and Gleason score and other prognostic factors were statistically investigated. Results: With E-cadherin, 47 (30%) of 156 cases showed loss of expression. There was a statistically significant difference between loss of e-cadherin expression and high Gleason score and seminal vesicle invasion. Expression loss was detected in 79 (50%) of 156 cases with beta-catenin. A statistically significant relationship was found between loss of beta-catenin expression and high Gleason score, seminal vesicle invasion and extraprostate spread. Conclusion: In our study, a statistically significant difference was found between E-cadherin and beta-catenin expression loss and high Gleason score, seminal vesicle invasion and extra-prostate spread. The findings supported the role of these molecules in the pathogenesis of prostate carcinoma. However, due to the absence of metastatic tumors and their relationship with clinical stage and clinical follow-up, no comment could be interpreted for the role of these molecules in aggressive tumors.

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