Deneysel Parsiyel Hepatektomi Modelinde Yorucu Egzersizin Karaciğer Rejenerasyonu Üzerine Etkisi

Amaç: Bu çalışmada amacımız yorucu egzersizin karaciğer rejenerasyonu üzerine etkisini parsiyel hepatektomi yapılan bir hayvan modeli üzerinde incelemektir. Gereç ve Yöntem: Çalışmaya ağırlıkları 225-275 g olan 24 Wistar albino sıçan dahil edildi. Deney grubuna (n=12) %70 hepatektomiden sonra iki defa yorucu egzersiz yaptırılırken; kontrol grubuna (n=12) herhangi bir egzersiz yaptırılmadı. Ardından deneklerin hepsinde parsiyel hepatektominin 72 saat sonrasında remnant karaciğerin rezeksiyonu gerçekleştirildi. Gruplar rejenerasyon indeksi, histopatolojik incelemede gözlenen rejeneratif değişiklikler, Ki-67 boyanma yoğunlukları ve mitoz sayısı açısından karşılaştırıldı. Bulgular: Gruplar, parsiyel hepatektomi ile elde edilen örneklerde yapılan incelemede rejeneratif değişiklikler benzer bulundu. Ki-67 boyanma yoğunluğu ve mitoz sayıları yönünden de gruplar arasında anlamlı farklılık saptanmadı. Egzersiz sonrasında sıçanların rejenerasyon indeksleri deney grubunda 1,93±0,34; kontrol grubunda ise 2,42±0,21 olarak hesaplandı (p=0,001). Gruplar arasında total hepatektomi sonrasında ölçülen rejeneratif değişiklik puanları (p=0,842) ve Ki-67 boyanma yoğunlukları (p=0,200) bakımından istatistiksel fark saptanmadı. Ancak mitoz sayıları, kontrol grubuna göre deney grubunda daha düşük olarak hesaplandı (11,5’e karşı 26,0; p=0,010). Sonuç: Bu sonuçlar ışığında yorucu egzersizin modelinde oluşturulan karaciğer rejenerasyonu hızını mitoz sayısı ve kütle artışı parametreleri bakımından olumsuz etkilediği, rejeneratif değişiklikler ve Ki-67 boyaması bakımından ise anlamlı olarak etkilemediği belirlendi. Konuyla ilgili daha detaylı plazma incelemeleri ve immünohistokimyasal analizler yapılarak daha nesnel ve daha geniş kapsamlı ve keskin sonuçlar sağlamak için ve farklı egzersiz yöntemleriyle egzersizin etkilerini ortaya koymak için konuyla ilgili daha ileri araştırmalar gerekmektedir.

Effect of Exhaustive Exercise on Liver Regeneration in an Experimental Partial Hepatectomy Model

Objectives: To investigate the results of exhaustive exercise on hepatocyte hyperplasia in an animal experiment consisting of partial hepatectomy. Materials and Methods: The experimental study was conducted with 24 Wistar Albino rats weighing between 225 and 275. Whereas exhaustive exercise was performed twice after 70% hepatectomy in the experimental group (n=12); the control group (n=12) did not perform an exercise. Then, rats underwent total hepatectomy 72 hours after partial hepatectomy. Groups were compared in terms of regeneration index, regenerative changes in hepatocytes, mitosis, and Ki-67 staining levels. Results: The groups were similar at baseline (at the time of 70% liver resection) regarding regenerative changes, mitosis count, and Ki-67. Regeneration index after exercise were 2.02±0.40 in the experimental group; and 2.42±0.21 in the control group (p=0.007). There was no difference between the groups in terms of regenerative scores (p=0.842) and Ki-67 staining (p=0.200) measured after total hepatectomy. However, mitosis was lower in the experimental group than the control group (11.5 vs. 26.0; p=0.010). Conclusion: It was showed that effect of exhaustive exercise on liver regeneration is negative according to mitosis count and liver mass increase but did not affect histologic changes of regeneration and Ki-67 staining. Blood tests and detailed immunohistochemical studies may reveal mechanisms of effect of exercise on liver cell hyperplasia and its potential clinical benefits.

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