Serdar DEMİR, Yalçın ERZURUMLU, İsmail ÖZTÜRK, Petek BALLAR KIRMIZIBAYRAK, Canan KARAALP

PTILOSTEMON CHAMAEPEUCE (L.) LESS.’İN SİTOTOKSİK VE ANTİMİKROBİYAL AKTİVİTESİ

Amaç: Bu çalışmanın amacı, P. chamaepeuce (L.) Less. (Asteraceae)’nin topraküstü kısımlarından hazırlanan n-hekzan, kloroform ve metanol ekstrelerinin antimikrobiyal ve sitotoksik aktivitelerinin araştırılmasıdır.Gereç ve Yöntem: Ekstrelerin antimikrobiyal aktiviteleri, standart bakteri kökenleri (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa, Bacillus subtilis ve Streptococcus pneumoniae) ve mantar kökenleri (Candida albicans ve C. parapsilosis) üzerinde mikrodilüsyon metodu ile araştırılmış ve minimum inhibitör konsantrasyon (MİK) değerleri belirlenmiştir. Ekstrelerin sitotoksik aktivitesi, WST-1 reaktifi kullanılarak hücre proliferasyon analiz yöntemi ile HeLa, U2OS, PC3, MCF-7 ve A549 olmak üzere farklı kanser hücre hatları üzerinde incelenmiştir.Sonuç ve Tartışma: Bitkiden elde edilen n-hekzan, kloroform ve metanol ekstrelerinin MİK değerlerinin S. aureus, E. faecalis ve B. subtilis’e karşı 250-1000 µg/mL konsantrasyon aralığında olduğu saptanmıştır. n-hekzan ve kloroform ekstrelerinin tüm kanser hücre hatlarında ise değişen düzeyde sitotoksik aktiviteye sahip oldukları belirlenmiştir (IC50: 21.0-67.1 μg/mL).

Anahtar Kelimeler:

Ptilostemon chamaepeuce, Antimikrobiyal aktivite, Sitotoksisite

CYTOTOXIC AND ANTIMICROBIAL ACTIVITIES OF PTILOSTEMON CHAMAEPEUCE (L.) LESS.

Objective: The aim of this study is to investigate the antimicrobial and cytotoxic activities of n-hexane chloroform and methanol extracts prepared from the aerial parts of P. chamaepeuce (L.) Less. (Asteraceae).Material and Method: The antimicrobial activities of the extracts on standard bacterial strains (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa, Bacillus subtilis and Streptococcus pneumoniae) and fungal strains (Candida albicans and C. parapsilosis) were examined by microdilution method and minimum inhibitory concentration (MIC) values were determined. The cytotoxic activity of the extracts was investigated by cell proliferation assay using WST-1 reagent on various cancer cell lines including HeLa, U2OS, PC3, MCF-7, and A549 cancer cell lines.Result and Discussion: The MIC values of n-hexane, chloroform and methanol extracts were between 250-1000 µg/mL against S. aureus, E. faecalis and B. subtilis. n-hexane and chloroform extracts had varying levels of cytotoxic activity in all tested cancer cell lines (IC50: 21.0-67.1 μg/mL).

Keywords:

Antimicrobial activity, cytotoxicity, Ptilostemon chamaepeuce,

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