Aslı KOC, Ekin KURTUL, Arzu Zeynep KARABAY, Mehmet TEKİN, Özlem BAHADIR ACIKARA

ALCHEMILLA MOLLIS (BUSER) ROTHM. İLE BİLEŞENLERİ HİPEROZİT VE İZOKERSETİNİN SİTOTOKSİK VE ANTİOKSİDAN AKTİVİTELERİ

Amaç: Alchemilla mollis (Buser) Rothm. (Rosaceae) Avrupa, Kuzey Anadolu, Kafkasya ve Kuzey İran’da geniş yayılış göstermektedir. A.mollis’in toprak üstü kısımlarından elde edilen ticari ilaç "Herba Alchemillae" Bulgar ve Türk halk tıbbında aşırı adet görme ve yara tedavisinin yanı sıra anstrenjan, diüretik, antispazmodik özellikleri için de kullanılmaktadır. Yapılan çalışmalarda A.mollis’te hiperozit, izokersetin, mikuelianin, cis- ve trans-tilirozit, sinokrassozit D2 ve rodiolgin tespit edildiği bildirilmiştir. Bu çalışmanın amacı A.mollis’in metanollü ektresi ile içerdiği bileşikler, hiperozit ve izokersetinin K562 lösemi hücre hattındaki sitotoksik aktiviteleri ile in vitro antioksidan aktivitelerini değerlendirmektir. Gereç ve Yöntem: Sitotoksik aktiviteyi test etmek için MTT testi, antioksidan aktiviteyi değerlendirmek için NO radikali süpürücü test kullanılmıştır. Sonuç ve Tartışma: Çalışma sonuçları A.mollis’in metanollü ekstresinin 0.02 mg/ml'den daha yüksek konsantrasyonlarda K562 hücrelerinin hücre canlılığını azaltırken, bileşiklerin aynı konsantrasyon aralığında herhangi bir sitotoksisite sergilemediğini göstermiştir. Ayrıca, test edilen tüm materyallerin NO radikali üretimini önemli ölçüde inhibe ettiği görülmüştür.

Anahtar Kelimeler:

Alchemilla mollis, antilösemik aktivite, antioksidan aktivite, hiperozit, izokersetin

CYTOTOXIC AND ANTIOXIDANT ACTIVITIES OF ALCHEMILLA MOLLIS (BUSER) ROTHM. AND ITS CONTITUENTS; HYPEROSIDE AND ISOQUERCETIN

Objective: Alchemilla mollis (Buser) Rothm. (Rosaceae) is widely distributed in Europe, North Anatolia, Caucasus, North Iran. A commercial drug, "Herba Alchemillae" is obtained from aerial parts of A. mollis, is used for its astringent, diuretic, antispazmodic properties as well as for treatment of excessive menstruation and wounds in Bulgarian and Turkish folk medicine. Previous studies have reported that hyperoside, isoquercetin, miquelianin, cis-and trans-tiliroside, sinocrassoside D2 and rhodiolgin were detected in A. mollis. Present study is aimed to evaluate in vitro antioxidant effects and cytotoxic activities of A.mollis methanolic extract and its constituents, hyperoside and isoquercetin, on K562 leukemia cell line. Material and Method: Spectrophotometric MTT assay and NO radical scavenging assay have been used to test the cytotoxic effects and antioxidant activities, respectively. Result and Discussion: Results showed that A.mollis methanolic extract reduced cell viability of K562 cells at concentrations higher than 0.02 mg/ml whereas the compounds did not exhibit any cytotoxicity at same concentration range. Additionally, significant inhibition on NO radical production was observed for all tested materials.

Keywords:

Alchemilla mollis, antileukemic activity, antioxidant activity, hyperoside, isoquercetin,

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