Fikret ŞILBIR, Mehmet KIZILKAYA, İlhami GÜLÇİN, Hüsnü KÜRŞAD, Nazım DOĞAN

Midazolamin antioksidan aktivitesi var mıdır?

Çalışmamızda, ratlarda midazolam sedasyonu sırasındaki oksidatif durum değerlendirilmiştir. Çalışmada 30 sıçan kullanıldı. Her bir grupta (kontrol, E vitamini ve midazolam) 10 sıçan vardı. Kontrol grubunda 5 mL $kg^-1$ $st^-1$ iv % 0.9 NaCl, E vitamini grubunda 100 mg $kg^-1$ iv E vitamini ve Midazolam grubunda 0.1 mg $kg^-1$ $st^-1$ iv. midazolam uygulandı. Kan örnekleri bazal dönemde ve çalışmanın birinci ve ikinci saatlerinde oksidatif aktivite ölçümleri için femoral ven ve arter kanülleri vasıtasıyla alındı ve malondialdehit, katalaz, peroksidaz, glutatyon redüktaz ve glukoz-6-fosfat dehidrogenaz ölçümleri yapıldı. Katlardan alınan kan örneklerinde midazolam ve E vitamini gruplarında kontrol grubu ile karşılaştırıldığında birinci ve ikinci saatlerde katalaz, peroksidaz, glutatyon redüktaz enzimlerinde artma, MDA düzeyinde azalma ayrıca G6PD enziminde ikinci saatte artma saptandı. Biz çalışmamızda hem midazolam hem de E vitamini ile birinci ve ikinci saatlerde anlamlı antioksidan aktivite artışı gözledik. Hem midazolamla hem de E vitamini ile oluşan antioksidan aktivitenin gücünün benzer olduğu sonucuna vardık.

Anahtar Kelimeler:

Midazolam, Vitamin E, Sıçan, Sprague-Dawley, Antioksidanlar

Does midazolam have an antioxidant activity?

in this study, ive evaluated the circulating oxidative status during sedation with midazolam in rats. Thirty rats were used in the study. in each group (control, vitamine E and midazolam groups) consisted of 10 rats. Control group received 5 mL $kg^-1$ $h^-1$ of iv 0.9 % NaCl. Vitamine E group received 100 mg $kg^-1$ of iv vitamine E . Midazolam group received 0.1 mg $kg^-1$ $h^-1$ of iv midazolam. Blood samples for oxidative activity measurements malondialdehyde, catalase, peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase were collected via a femoral vein and arterial catheterfor baseline, $1^st$ and $2^nd$ hours. Catalase, peroxidase, and glutathione reductase increased, MDA decreased at l and 2 hrs. also, G6PD increased at 2 hrs were detected in blood of rats with treated vitamine E and midazolam as compared with control. Wefound a significant antioxidant activity at lst and $2^nd$ hours in the blood in both midazolam and vitamine E groups, we conculuded that, power of antioxidant activity were similar with midazolam and vitamine E.

Keywords:

Midazolam, Vitamin E, Rats, Sprague-Dawley, Antioxidants,

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