Aykut BOSTANCI, Aytaç KOCABAŞ, Yasin UZUN, Gökhan SADİ, Sevim KOL

Antioksidan ve antimiktobiyal özellikleri ile birlikte Craterellus cornucopioides (L.) Pers. türünün hücre büyümesi baskılama potansiyeli

Ölüm trompeti veya bolluk boynuzu olarak da bilinen Craterellus cornucopioides (L.) Pers, yenilebilir bir makrofungustur. Bu çalışma Craterellus cornucopioides (L.) Pers mantarının sağlık açısından yararlı özelliklerini açığa çıkarmak amacıyla yapılmıştır. Çalışmada ilgili mantarın biyoaktif içerikleri (fenolikler, flavonoidler, β-karoten ve likopen) ve DPPH radikal süpürücü aktiviteleri belirlenmiştir. Ek olarak, HepG2 hücreleri ve bazı bakteri türleri üzrine hücre büyümesini baskılayıcı etkileri değerlendirilmiştir. Buna göre, su ve metanol ekstraktları sırasıyla 37.71±1.42 μg/mg ve 13.78±1.60 μg/mg fenolik içeriğe sahiptir. Benzer şekilde, metanol ekstraktları sulu ekstrelere kıyasla daha yüksek β-karoten ve likopen içeriğine sahiptir. Bu antioksidanlara paralel olarak, metanol ekstraktları da DPPH süpürme aktivitesine daha fazla sahiptir (ICso: 5.26±0.67 mg/ml). Ayrıca su ekstraktları, metanol ekstraklarına göre daha yüksek flavonoid içeriğine (2.13±0.06 μg/mg) sahiptir. C. cornucopioides, HepG2 hücresi üzerinde de önemli bir hücre büyümesi engelleyici etkiye sahiptir (IC50: su ekstratları için 18.41±1.10 mg/ml ve metanol ektraktları için IC50: 3.14±1.07 mg/ml). Buna ek olarak, her iki ekstrakt da test edilen altı farklı bakteri türü üzeine etkili olmuştur. Sonuç olarak, bu çalışma C. cornucopioides'in yüksek antioksidan bileşenleri nedeniyle hücresel oksidatif stresi azaltabildiğini, patojen mikroorganizmalarının büyümesini inhibe edebileceğini ve en azından HepG2 hücrelerine bir miktar hücre büyümesi inhibitör potansiyeline sahip olduğunu göstermektedir.

Cell growth inhibitory potential of Craterellus cornucopioides (L.) Pers. together with antioxidant and antimicrobial properties

Craterellus cornucopioides (L.) Pers which is alsoknown as trumpet of death or horn of plenty, is a wild edible macrofungus. Thisstudy was conducted to elucidate the potential health beneficial properties of C. cornucopioides. Bioactive ingredients(phenolics, flavonoids, β-carotene and lycopene) and DPPH radical scavengingactivities were determined. Additionally, cell growth inhibitory effects on HepG2 cells togetherwith some bacteria were evaluated. Accordingly, water and methanol extractscontains 37.71±1.42 μg/mg and 13.78±1.60 μg/mg phenolic contents, respectively.Similarly, methanolic extracts have higher β-caroten and lycopene content ascompared to aqueous extracts.In parallel with these antioxidants, methanolic extracts have also higher DPPHscavenging activity (IC50: 5.26±0.67 mg/ml). Besides, water extractshave higher flavonoid contents (2.13±0.06 μg/mg) then the methanolicextracts. C. cornucopioides hasalso an important cell growth inhibitory effects on HepG2 cell (IC50:18.41±1.10 mg/ml for aqueous extracts and IC50: 3.14±1.07 mg/ml formethanolic extracts). Moreover, both extracts were effective on six differentbacteria tested. As a result, this study indicates that C. cornucopioides could reduce thecellular oxidative stress because of its high antioxidant ingredients, inhibitthe growth of pathogen microrganisms and have some degree of cell growthinhibitory potential at least to the HepG2 cells.Pers which is (L.) Craterellus cornucopioides

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