Verbascum scamandri Murb. (Scrophulariaceae) Türünün Çekirdek DNA İçeriğinin ve Kromozom Sayısının Belirlemesi

Çalışmanın amacı, endemik bir bitki olan Verbascum scamandri türünün in vitro büyütülen 14 haftalık bitkiler kullanılarak kromozom sayısı ve çekirdek DNA içeriğinin belirlenmesi, 2 mg/L Kin + 0.1 mg/L 2,4-D içeren MS ortamında çoğaltılan 9 haftalık in vitro bitkilerde ve 1 mg/L Kin + 1 mg/L 2,4-D içeren MS ortamında indüklenen kallus dokularında flow sitometri analizleri ile genetik kararlılığın tespit edilmesidir. Mitoz kromozom sayımlarında türün kromozom sayısının 2n = 32 olduğu belirlenmiştir. Flow sitometri analizinde, in vitro yetiştirilen bitki örneklerinin 0.73 pg/2C ± 0.01, kallus örneklerinin 0.76 pg/2C ± 0.02, in vitro çoğaltılmış bitkiciklerin DNA içeriği ise 0.79 pg/2C ± 0.01 belirlenmiştir. Yapılan flow sitometri analizleri tohumdan gelişen bitkiler ile in vitro çoğaltılan bitkilerin benzer çekirdek DNA içeriklerine sahip olduklarını göstermiş ve dolayısıyla genetik stabil oldukları anlaşılmıştır.

Anahtar Kelimeler:

Çekirdek DNA İçeriği, Kromozom, Doku Kültürü, Sığırkuyruğu

Determination of Nuclear DNA Content and Chromosome Number of Verbascum scamandri Murb. (Scrophulariaceae)

The study aimed to determine the chromosome number and nuclear DNA content of an endemic plant, Verbascum scamandri, using 14-week-old plants germinated from seed and to determine the genetic stability based on flow cytometry analyses in callus tissue induced MS medium containing 1 mg/L Kin + 1 mg/L 2,4-D and 9-week-old propagated plants, induced and developed on MS medium containing 2 mg/L Kin + 0.1 mg/L 2,4-D. In the mitotic chromosome counts showed that V. scamandri had 2n = 32 chromosome number. Flow cytometric analysis revealed that 14-week-old in vitro grown plants have 0.73 pg/2C ± 0.01, callus tissue has 0.76 pg/2C ± 0.02 and propagated plantlets have 0.79 pg/2C ± 0.01. The results proved that propagated plants had similar DNA content to the seed-derived plants which showed analysed plants were genetically stable.

Keywords:

Nuclear DNA Content, Chromosome, Tissue Culture, Mullein,

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