Harun BAYRAKTAR, Kübra TEKİN, Göksel ÖZER

SOĞAN ÜRETİMİ İLE İLİŞKİLİ FARKLI FUSARİUM TÜRLERİNİN PCR-RFLP ANALİZİ

Bu çalışma kapsamında ülkemizde soğan üretimi ile ilişkili farklı Fusarium türleri arasındaki genetik akrabalık PCR-RFLP yöntemi ile araştırılmıştır. F. oxysporum, F. solani, F. acuminatum, F. equiseti, F. proliferatum, F. redolens ve F. culmorum’ u temsil eden 42 fungus izolatının ribozomal DNA ITS bölgesi ITS1/4 primerleri ile çoğaltıldıktan sonra HinfI, AluI, MspI, EcoRI restriksiyon enzimleri ile kesilmiştir. PCR analizi sonucunda tüm izolatlardan 550-570 bp büyüklüğünde tek bir DNA fragmenti çoğaltılmıştır. Enzim kesimleri ise Fusarium türlerinin birbirinden ayrılmasını sağlayan 17 farklı enzim profili oluşturmuştur. ITS bölgesinin RFLP analizi ile oluşturulan dendogramda tüm Fusarium türleri farklı gruplar içerisinde yer almıştır. Fusarium türleri arasındaki benzerlik oranının ise 0.70-0.13 arasında değiştiği tespit edilmiştir. Elde edilen sonuçlar enzim profillerinin soğan üretim alanlarında ekonomik olarak önemli kayıplara yol açan Fusarium türlerinin ayrılmasında oldukça faydalı olduğunu göstermiştir.

PCR-RFLP ANALYSIS OF DIFFERENT FUSARIUM SPECIES ASSOCIATED WITH ONION PRODUCTION

The study analyzed genetic relationships among different Fusarium species associated with onion production of Turkey using PCR-RFLP technique. ITS region of ribosomal DNA of 42 fungal isolates, representing F. oxysporum, F. solani, F. acuminatum, F. equiseti, F. proliferatum, F. redolens, and F. culmorum was amplified with primer pairs ITS1/4 and digested with HinfI, AluI, MspI, EcoRI enzymes. PCR amplification yielded a single DNA fragment ranging in size from 550-570 bp from all isolates. Restriction digests revealed 17 restriction patterns, providing the differentiation of Fusarium species from each other. All Fusarium species clustered into different groups in the dendogram derived from the data of PCR-RFLP. Similarity index ranged from 0.70 to 0.13 among Fusarium species. The results revealed that restriction patterns were very useful in the discrimination of Fusarium species, causing economically significant losses in onion production areas.

Keywords:

Onion Fusarium spp., ITS, PCR-RFLP,

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