Pestisit klorprifosun rat (wistar albino) karaciğerinde oluşturduğu hasar üzerine kurkuminin antioksidan etkisinin incelenmesi

Bu çalışmada dünyada yaygın olarak kullanılan geniş spektrumlu pestisit olan klorprifosun (CPF) ratların (Wistar albino) karaciğerinde oluşturduğu hasarın miktarı ve bu hasarın giderilmesinde antioksidan özelliği bilinen kurkuminin koruyucu rolünün incelenmesi amaçlanmıştır. Bu amaçla, bir kontrol grubu ile sırasıyla sadece CPF, sadece kurkumin ve CPF+kurkumin verilen 3 muamele grubu olmak üzere toplam dört grup oluşturulmuştur. Her bir grupta 12 adet 90 günlük erkek rat kullanılmıştır. 5 mg/kg/gün CPF ve 100 mg/kg/gün kurkumin gavaj yoluyla hayvanlara verilmiştir. 15. ve 30. günlerde gruplardaki 6’şar hayvan sakrifiye edilmiştir. Histolojik takip işlemlerinden sonra ışık mikroskobu ile yapılan değerlendirmede CPF grubunda kontrol grubuyla karşılaştırıldığında karaciğerde hidropik dejenerasyon, inflamasyon odakları, sinüzoidlerde genişlemeler, hepatositlerde sitoplazmik değişiklikler, glikojen yoğunluğunda farklılıklar gözlenmiştir. CPF ile birlikte kurkumin verilen grupta, CPF grubuna göre daha az oranda histolopatolojik değişiklik gözlenmiştir. Elde edilen bulgular kurkuminin, CPF’nin neden olduğu histopatolojik hasarı indirgediğini göstermektedir.

Investigation of curcumin’s antioxidant effect on pesticide chlorpyrifos caused damage in rat (Wistar albino) liver

In this study, broad-spectrum pesticide chlorpyrifos (CPF) caused damage on rat (Wistar albino) liver and protective role of curcumin, a well known antioxidant were investigated. For this purpose, 4 groups were created, including a control group with 3 treatment groups; only CPF, only curcumin and CPF + curcumin, respectively. Each group was consisted of 12 male rats. 5 mg/kg/day CPF and 100 mg/kg/day curcumin were given to the animals via gavage. 6 animals in each group were sacrificed in 15th and 30th days. Following histologic examinations, when the CPF group compared with the control group; hydropic degenerations, focal inflammatory cell infiltration, expansion in the sinusoidal, cytoplasmic changes in hepatocytes and glycogen concentration differences were observed by light microscopic evaluations. It was observed that CPF + curcumin-treated group had histological changes than CPF group. The results showed that curcumin has a reduction effects on CPF induced histopathological damage.

Keywords:

Histological changes Liver, Chlorpyrifos, Curcumin,

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