Kadmiyumun Küçük Balmumu Güvesi Larvalarında Protein, Lipit ve Karbohidrat Miktarları ile Etkileşimi

Kadmiyumun küçük balmumu güvesi, Achroia grisella Fabricius, 1794 (Lepidoptera: Pyralidae) larvalarının protein, lipit ve karbohidrat miktarlarına etkisi laboratuvar koşullarında araştırılmıştır. Larvalar kadmiyumun üç farklı konsantrasyonu (50, 100 ve 200 mg kg-1 besin) ile kontamine edilmiş yapay besin ortamında yetiştirildi. Kontrol grubundaki böcekler bal peteği (200 g), kepek (860 g), gliserol (300 ml), bal (150 ml) ve saf su (150 ml) içeren ancak kadmiyum içermeyen besinle beslendi. Böceklerin tümü 25 ± 2 °C sıcaklık, % 60 ± 5 bağıl nem ve 16A: 8K fotoperyot koşullarında yetiştirildi. Biyokimyasal analizler için son evre A. grisella larvaları tartılıp ayrı mikrosantrifüj tüplerine konularak -20 °C'de tutuldu. Bu çalışmanın sonuçları 50 ve 200 mg kg-1 kadmiyum konsantrasyonlarında kontaminasyonun larvaların protein seviyesinde önemli bir düşmeye neden olduğunu göstermiştir. Karbohidrat miktarı sadece 50 mg kg-1 kadmiyum konsantrasyonunda kontrol ile karşılaştırıldığında önemli ölçüde azalmıştır. Kadmiyum uygulanan larvaların lipit miktarı, 50 ve 200 mg kg-1 kadmiyum konsantrasyonlarında çok değişmediği halde, 100 mg kg-1 da önemli ölçüde düşmüştür.

The Interaction of Cadmium with Protein, Lipid and Carbohydrate Concentrations in Lesser Wax Moth Larvae

The influence of cadmium on protein, lipid and carbohydrate levels of lesser wax moth, Achroia grisella Fabricius, 1794 (Lepidoptera:Pyralidae) larvae was examined under laboratory conditions. Larvae were reared on an artificial diet medium contaminated with three different concentrations of cadmium (50, 100 and 200 mg kg-1 diet). In the control group the insects fed on cadmium-free diet containing honeycomb (200 g), bran (860 g), glycerol (300 ml), honey (150 ml) and distilled water (150 ml). All of the insects were kept at 25 ± 2 °C temperature, 60 ± 5 % relative humidity and 16L:8D photoperiod conditions. Last instar A. grisella larvae were weighed, placed individually into microcentrifuge tubes and stored at –20 °C for the biochemical analysis. The results of this study showed that cadmium contamination caused a significant reduction in the protein level of the larvae at 50 and 200 mg kg-1 concentrations. Carbohydrate level reduced significantly at only 50 mg kg-1 cadmium concentration compared with control. Lipid level of the cadmium-treated larvae considerably reduced at 100 mg kg-1 concentration, while it remained more or less unaffected at 50 and 200 mg kg-1 cadmium concentrations.

Keywords:

Achroia grisella Biochemical composition, Heavy metal,

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