Akciğer Mast Hücre Davranışının Migren Sıçan Modelinde İncelenişi: Migren Baş Ağrısı İçin Çıkarımlar

Amaç: Bu çalışmada sıçanlarda nitrogliserin ile oluşturulan migren modeli kullanılarak akciğer mast hücrelerinin migrendeki muhtemel rolünü araştırmak amaçlanmıştır. Gereç ve Yöntemler: Otuz beş yetişkin erkek Wistar sıçan beş gruba bölündü. Tüm uygulamalar intraperitoneal yoldan olmak üzere; NS grubuna 0,2 ml normal salin (serum fizyolojik), NTG grubuna 10 mg/kg nitrogliserin, vehikül NTG grubuna 0,2 ml normal salin içinde %0,1’lik etanol, L-NAME+NTG grubuna 50 mg/kg L-NAME + nitrogliserin, NS+NTG grubuna ise 0,2 ml normal salin + nitrogliserin uygulandı. Nitrogliserin ve diğer uygulamalardan iki saat sonra sıçanlar anestezi altında 150 ml %4’lük paraformaldehit ile intrakardiyak olarak perfüze edildi. Akciğerler alındı ve mast hücrelerini gözlemlemek için toluidin mavisi ile boyandı. Veriler tek yönlü varyans analizi ile incelendi. Bulgular: Nitrogliserin akciğer mast hücrelerinin hem sayısında (94±3,8’den 131±6,7’ye; p=0,0035) hem de degranülasyon yüzdesinde (%11,8±1,3’ten %35,5±5,7’ye; p=0,003) anlamlı bir artış sağladı. L-NAME ise akciğer mast hücrelerinin sayı ve degranülasyonu üzerinde nitrogliserinin yol açtığı bu etkileri önledi, böylece akciğer mast hücrelerinin nitrogliserin ile tetiklenerek artan sayısını ve degranülasyon yüzdesini ayrı ayrı azalttı. Tartışma ve Sonuç: Bulgularımız nitrogliserin ile oluşturulan migren sıçan modelinde nitrogliserin uygulamasının akciğer mast hücrelerinin sayısında ve degranülasyonunda bir artışa yol açtığını göstermiştir. Ayrıca L-NAME’nin bu etkileri önlemiş olması, nitrogliserin kaynaklı nitrik oksidin direkt veya indirekt olarak akciğer mast hücrelerinin aktivasyonuna yol açtığını düşündürmektedir. Bu çalışmada elde edilen sonuçlar akciğer mast hücrelerinin migrendeki rolü üzerine yapılacak yeni araştırmalara öncülük edebilir.

An Investigation of Lung Mast Cell Behavior in a Rat Model of Migraine: Implications for Migraine Headache

Aim: In this study, we aimed to investigate the potential role of lung mast cells in migraine by using anitroglycerin-induced migraine model in rats.Materials and Methods: Thirty-five adult male Wistar rats were divided into five groups. Each treatmentbeing performed intraperitoneally, the NS group received 0.2 ml normal saline, the NTG group 10 mg/kgnitroglycerin, the NTG vehicle group 0.2 ml 0.1% ethanol in normal saline, the L-NAME+NTG group 50mg/kg L-NAME + nitroglycerin, and the NS+NTG group normal saline + nitroglycerin. Two hours after thenitroglycerin and other treatments, the rats under anesthesia were intracardially perfused with a solutionof 150 ml 4% paraformaldehyde. The lungs were harvested and stained with toluidine blue to observemast cells. The data were analyzed by using one-way ANOVA.Results: Nitroglycerin increased significantly both number (from 94±3.8 to 131±6.7, p=0.0035) and percentof degranulation (from 11.8±1.3% to 35.5±5.7%, p=0.003) of lung mast cells. However, L-NAMEprevented the effects of nitroglycerin on the number and degranulation of lung mast cells, attenuatingthe increase in both number (from 136±5.1 to 93±2.5, p=0.0011) and degranulation percentage of lungmast cells (from 37.3±1.0% to 13.6±3.1%, p=0.001) induced by nitroglycerin.Discussion and Conclusion: Our findings demonstrate that nitroglycerin treatment leads to an increasein the number and degranulation of lung mast cells in a rat model of migraine induced by nitroglycerin.Moreover, L-NAME prevented these effects, suggesting that nitric oxide released from nitroglycerin directlyor indirectly leads to the activation of lung mast cells. The results obtained in the current study canpioneer new research on the role of lung mast cells in migraine.

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