Vanadyuma Maruz Kalan Buğday Genomunda DNA Metilasyon Düzeyinin CRED-RA Tekniği Kullanılarak Araştırılması

Vanadyum (V), endüstride yaygın kullanımı nedeniyle ciddi bir kirletici haline gelmiştir. Bu çalışmada, Triticumaestivum L. ‘Çetinel 2000’ tohumlarındaki DNA metilasyon değişiklikleri CRED-RA (Çift Restriksiyon Enzimi Kesimi veRastgele Çoğaltım) analizleri ile belirlenmiştir. DNA hasarları ve genomik kararsızlık Basit Tekrarlı Diziler Arası Polimorfizm (ISSR) analizi ile belirlenmiştir. Vanadyum çözeltileri (4,4 mM, 6,6 mM, 8,8 mM) ve borik asit (BA) çözeltileri (4 mM ve 8 mM) kullanılmıştır. CRED-RA analizine göre, V uygulamasının buğday genomunda DNA metilasyon seviyesinde BA uygulamasına göre daha fazla değişikliğe neden olduğu görülmüştür. Tüm V dozları DNA hasarına sebep olarak genomik kararlılığın (GTS) azalmasına neden olmuştur. V ile birlikte uygulanan BA dozlarında genomik kararlılık korunmuştur. Sonuç olarak, BA'nın V stres koşulları altında bitkilerde genetik hasarı azaltmak için alternatif bir kimyasal olabileceği düşünülmektedir.

Investigation of DNA Methylation Level in Wheat Genome Exposed to Vanadium by Using CRED-RA Technique

Vanadium (V) has become a serious pollutant due to its widespread use in industry. In this study, DNA methylation changes in Triticum aestivum L. 'Çetinel 2000' seeds were determined by CRED-RA (Coupled Restriction Enzyme Digestion-Random Amplification) analysis. DNA damage and genomic instability were determined by Simple Repeat Inter-Sequence Polymorphism (ISSR) analysis. Vanadium solutions (4.4 mM, 6.6 mM, 8.8 mM) and boric acid (BA) solutions (4 mM and 8 mM) were used. According to CRED-RA analysis, it was observed that V caused more changes in the DNA methylation level in wheat genome compared to BA application. All doses of V caused DNA damage and decreased genomic template stability (GTS). It was determined that the genomic stability was maintained in BA doses applied with V. Genomic stability was maintained at BA doses administered with V. As a result, BA may be an alternative to reduce genotoxic damage to V stress in plants.

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