The Potential of Orange Peel Oil as a Suppressor of Cell Proliferation in Animal Feed and Human Nutrition: An Experimental Study

This study aimed to investigate the in vitro cytotoxic activities of orange peel oil on HaCaT cell lines by using an MTT cytotoxicity assay after administering orange peel oil at different doses and timepoints. Our objective was to assess the in vitro cytotoxic activities of orange peel oil on HaCaT cell lines. Cell viability was determined with the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazoliumbromide] assays. The HaCaT cells (100 µL) were cultured in plates and treated with different concentrations of orange peel oil (25 µM, 50 µM, 100 µM, 150 µM and 200 µM) for durations of 24 and 48 hours. Cell death was determined by collecting and staining with 0.4% Trypan blue for 5 minutes at room temperature, followed by microscopic examination. There was a significant difference between the doses concerning both time zones (p

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Alınteri Zirai Bilimler Dergisi-Cover
  • ISSN: 2564-7814
  • Başlangıç: 2007
  • Yayıncı: Adem Yavuz SÖNMEZ
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