Semir ÖZDEMİR, Nurettin OĞUZ, Nihal ÖZTÜRK ERBOĞA, Barış Özgür DÖNMEZ

Diyabetik Sıçanlarda Kemik Dokusunda Lipid Peroksidasyonu ve Antioksidan Sistem Üzerine Losartan’ın Etkileri

Amaç: Diyabet, kırık riskini artırabilecek osteopeni ve osteoporoz gibi kemik hastalıklarına yol açarken, serbest radikallerin diyabetle ilişkili kemik rezorpsiyonunda önemli rol oynadığı öne sürülmüştür. Anjiyotensin-dönüştürücü enzim inhibitörleri ve anjiyotensin reseptör blokörlerinin diyabetik hastalarda kemik kaybını ve kırılma riskini azaltabilmesi muhtemeldir. Bu nedenle çalışmamız, diyabetik sıçan femurlarında losartanın oksidatif stres ve antioksidan savunma sistemi üzerindeki etkilerini araştırmak ve böylece kemik kalitesindeki bozulmanın olası mekanizmasını açıklamak için tasarlanmıştır.Gereç ve Yöntemler: Sıçanlar dört gruba ayrılmıştır: Kontrol K , diyabet D , losartanla tedavi edilen diyabet D-LOS ve losartanla tedavi edilen kontrol K-LOS . Diyabet, tek doz streptozotosin 50 mg/kg enjeksiyonu ile indüklenmiştir. Diyabetli sıçanlara 8 hafta boyunca 5 mg/kg/gün losartan uygulanmıştır. Kemik dokularında, 2-tiobarbiturik asit reaktif ürünlerinin düzeyi TBARS , süperoksit dismutaz SOD , katalaz KAT ve glutatyon peroksidaz GPx aktiviteleri incelenmiştir.Bulgular: Diyabet uygulaması ile kemik dokuda anlamlı düzeyde artan TBARS değerleri losartan uygulaması ile düşmüştür. Diğer yandan, D grubunda SOD aktivitesi azalırken KAT aktivitesinde bir değişiklik gözlenmemiştir ve losartan tedavisi SOD aktivitesi üzerinde etkili olmamıştır. Diyabet ile birlikte anlamlı düzeyde düşen GPx aktivitesi losartan tedavisi ile normal seviyelerine dönmüştür.Sonuç: Bu bulgular, losartan tedavisinin diyabetik sıçan femurunda oksidatif stresin indüklediği kemik kalitesindeki düşüşü antioksidan enzimlerin modülasyonunu sağlayarak düzeltebileceğini göstermektedir

Anahtar Kelimeler:

Diyabet, Losartan, Oksidatif stres, Antioksidanlar

The Effects of Losartan on Lipid Peroxidation and the Antioxidant System in the Bone Tissue of Diabetic Rats

Objective: Diabetes leads to bone disorders such as osteopenia and osteoporosis that can increase the fracture risk and free radicals have been suggested to play significant role in the bone resorption associated with diabetes. Angiotensin-converting enzyme inhibitors and angiotensin receptor blockers may reduce bone loss and fracture risk in diabetic patients. This study was therefore designed to investigate the effects of losartan on bone quality and the antioxidant defense system in diabetic rat femurs.Material and Methods: The rats were divided into four groups: Control C , diabetes D , losartan-treated diabetes D-LOS and losartan-treated control C-LOS . Diabetes was induced by single injection of streptozotocin 50 mg/kg . The treated rats received 5 mg/kg/day of losartan for 8 weeks. Superoxide dismutase SOD , catalase CAT and glutathione peroxidase GPx activities and 2-thiobarbituric acid reactive substances TBARS levels were analyzed in bone tissue samples.Results: Diabetes revealed a highly significant increase in the TBARS values of rat bones and losartan administration significantly lowered the TBARS levels. SOD activity was decreased in group D but no change in CAT activity was observed and losartan treatment did not affect SOD activity. Diabetes resulted in a marked decrease in GPx that was increased significantly by losartan. Conclusion: These findings indicate that losartan can prevent bone loss and improve the quality of diabetic rat femurs, most probably to reduction of reactive oxygen species and/or modulation of antioxidant enzymes

Keywords:

Diabetes, Losartan, Oxidative stress, Antioxidants,

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