Fazile Nur EKİNCİ AKDEMİR, Ayhan TANYELİ, Ersen ERASLAN, Mustafa Can GÜLER, Ömer TOPDAĞI, Tuncer NACAR

Brusatol'ün Renal İskemi Reperfüzyon Hasarına Karşı Renoprotektif Etkileri

Amaç: Bu çalışmanın amacı brusatol tedavisinin bilateral renal iskemi reperfüzyon (RIR) yöntemi ile indüklenen oksidatif böbrek hasarı üzerindeki potansiyel yararlı etkilerini belirlemektir. Gereç ve Yöntem: Mevcut çalışmada, deney hayvanları rastgele sham, renal iskemi reperfüzyonu (RIR), DMSO ve brusatol grupları olarak 4 gruba ayrıldı. Sham grubu; arka bölge insizyon ile açıldı ve sonra sütüre edildi ancak iskemi reperfüzyon (IR) modeli oluşturulmadı. RIR grubunda 24 saatlik reperfüzyonu takiben 1 saatlik iskemi oluştu. DMSO grubunda, 10 gün boyunca iki günde bir kez her sıçan için 0,3 ml %1 DMSO intraperitonal olarak uygulandı ve reperfüzyondan 30 dakika önce son bir doz uygulandı. Daha sonra RIR grubunda anlatıldığı gibi IR modeli uygulandı. Brusatol grubunda brusatol, deneyden önce 10 gün boyunca her bir sıçan için 0,5 mg/ml olarak intraperitonal olarak uygulandı. Son doz reperfüzyondan 30 dakika önce uygulandı ve IR, RIR grubunda gösterildiği gibi gerçekleştirildi. Reperfüzyon döneminden sonra sıçanlar hareketsiz hale getirildi ve böbrek dokuları izole edildi. Bulgular: TNF-a, MDA ve IL-1β düzeyleri, OSI, TOS ve MPO değerleri anlamlı olarak yükselmiş, ancak TAS ve SOD düzeyleri sham grubuna göre RIR ve DMSO gruplarında azalmıştır. Öte yandan TAS ve SOD artarken OSI ve TOS değerleri, MPO ve TNF-α, MDA ve IL-1β düzeylerinin brusatol + I/R grubunda sham ve DMSO gruplarına göre brusatol tedavisine bağlı olarak anlamlı düzeyde azaldığı görülmüştür. Sonuç: Sonuç olarak, brusatol sıçanlarda RIR kaynaklı oksidatif böbrek hasarına karşı koruyucu etkiler göstermiştir.

Anahtar Kelimeler:

Brusatol, Renal İskemi Reperfüzyonu, Oksidatif stres, Sıçan.

Reno-protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury

Purpose: The aim of this study was to determine the potential beneficial effects of brusatol treatment on oxidative kidney injury induced by bilateral renal ischemia reperfusion (RIR) method. Material and Method: In the existing study, experimental animals were randomly assigned to 4 groups as sham, renal ischemia reperfusion (RIR), DMSO and brusatol groups. Sham group; the back region was opened by incision and then sutured but no ischemia reperfusion (IR) model was established. In RIR group, 1 hour of ischemia following 24 hours of reperfusion was formed. In DMSO group, 0,3 ml, 1% DMSO was administered intraperitoneally for each rat once in two 2 days for 10 days and a last dose was applied 30 minutes before reperfusion. Then IR model was carried out as told in RIR group. In brusatol group, brusatol was applied intraperitoneally as 0,5 mg/ml for each rat every second days for 10 days before the experiment. The last dose was administered 30 minutes before reperfusion and IR was fulfilled as depicted in RIR group. Following reperfusion period, rats were immolated and renal tissues were isolated. Results: TNF-α, MDA and IL-1β levels, OSI, TOS and MPO values were significantly raised but TAS and SOD levels were declined in RIR and DMSO groups compared to sham group. On the other side, TAS and SOD increased while OSI and TOS values, activity of MPO and TNF-α, MDA and IL-1β levels were significantly reduced in brusatol+I/R group due to brusatol therapy compared to sham and DMSO groups. Conclusion: Consequently, brusatol demonstrated protective effects against RIR induced oxidative kidney injury in rats.

Keywords:

Brusatol, Renal Ischemia Reperfusion, Oxidative stress, Rat.,

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