The antinociceptive effects of systemic administration of tramadol,gabapentin and their combination on mice model of acute pain

Amaç: Tek başına veya birlikte sistemik olarak verilen tramadol ve gabapentinin farelerde akut ağrıda olası antinosiseptif etkleri- ni araştırmaktır. Gereç ve Yöntem: Hayvan etik komitesinin onayı sonrası; 96 BALB/c albino dişi fare 12 gruba ayrıldı: (I) Enjeksiyon yapılmak- sızın kontrol grubu, (II) salin uygulanan kontrol grubu, (III)-(IV) tramadol 10 mg/kg veya 30 mg/kg uygulanan gruplar, (V)- (VIII) gabapentinin sırasıyla 30, 100, 200, 300 mg/kg uygulandığı gruplar idi. Tramadol ve gabapentinin olası etkileşimlerinin araştırıldığı gruplarda farelere tramadol+gabapentinin dört farklı dozları uygulandı; (30+30, 30+100, 30+200 ve 30+300 mg/ kg) (sırasıyla, Grup IX-XII). Farelerin her 10 gram ağırlığı için intraperitoneal olarak 0.1 mL ilaç uygulandı. Enjeksiyondan 30 da- kika sonra tail-fick ve hot plate testleri gerçekleştirildi. Bulgular: On ve 30 mg/kg tramadol tail-fick ve hot plate testlerinde doz bağımlı antinosiseptif etki oluşturdu. Gabapentin 300 mg/ kg dışındaki dozlarda tail-fick testinde antinosiseptif etki oluşturmazken, hot plate testinde doz bağımlı antinosiseptif etki oluştur- du. Her iki testte de tramadol ve gabapentin birlikte kullanıldıklarında, tek başlarına kullanılmalarına oranla daha fazla antinosi- septif etki oluşturdu, fakat sadece 30 mg/kg tramadol + 300 mg/kg gabapentin birlikte kullanıldığında her iki testte de anlamlı an - tinosiseptif etki saptandı (p

Farelerde akut ağrıda sistemik uygulanan tramadol, gabapentin ve kombinasyonlarının antinosiseptif etkileri

Objectives: Te aim of the present study was to investigate the possible antinociceptive efects of systemic administration of tramadol and gabapentin either alone or in combination on acute pain models in mice. Methods: After obtaining the approval of Animal Ethics Committee; 96 BALB/c albino male mice were divided into 12 groups: (I) control without injection, (II) control treated with saline, (III)-(IV) mice treated with tramadol 10 mg/kg or 30 mg/kg, (V)-(VIII) mice treated with gabapentin; 30, 100, 200, 300 mg/kg respectively. In order to determine possible interac- tions between tramadol gabapentin and; mice received four diferent combinations of tramadol + gabapentin (30+30, 30+100, 30+200 and 30+300 mg/kg) (Groups IX-XII respectively). Mice received 0.1 ml solution for every 10 g of their weight. Te drug was injected into peritonea. Tirty minutes after the drug injection, tail-fick and hot-plate tests were conducted. Results: Ten and 30 mg/kg tramadol produced dose dependent antinociceptive efect in tail-fick and hot plate tests. Gabap - entin had no antinociceptive efect in the tail fick test except 300 mg/kg dose, and had dose dependent antinociceptive efect in hot-plate test. In both tests, various combinations of tramadol and gabapentin produced an antinociceptive efect that is greater than that produced by tramadol and gabapentin alone. But, just 30 mg/kg tramadol + 300 mg/kg gabapentin combi- nation caused statistically signifcant increase in both tests (p<0.05). Conclusion: When gabapentin and tramadol were used in combination, gabapentin had no additive antinociceptive efect except for 300 mg/kg in tail-fick and hot-plate tests. Tail-fick test showed that tramadol produced better antinociceptive efect than gabapentin.

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Ağrı-Cover
  • ISSN: 1300-0012
  • Yayın Aralığı: Yılda 4 Sayı
  • Başlangıç: 2018
  • Yayıncı: Ali Cangül
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