Vitreoscilla Hemoglobin Geni (vgb) Taşıyan İki Farklı Plazmidin Citrobacter freundii’nin ATP Üretimi ve Oksijen Alınımına Etkileri

Bu çalışmada, ATP üretimi, oksijen alımı ve hücre kütlesi üretimi üzerinde yeniden birleştirici oksijen alımı sisteminin kullanılması araştırılmıştır. Citrobacter freundii ve onun vgb rekombinantları kullanılmıştır. Citrobacter freundii de geniş bir konakçı aralığında çalışan plazmitler Cf [PMK'yi 79] ve Cf [pUC8: 15] kullanılmıştır. En İyi ATP üretimi Cf [PMK 79] (0,17 mg/ g). Cf [pMK79] oksijen alımının seviyesi, Cf[pUC8:15] daha yüksektir. Oysa Cf [pMK79]'un OD600 değerleri 48 saat sonunda düşmektedir.

Effects of Citrobacter freundii ATP Production and Oxygen Intake Two Different Plasmids Carrying the Vitreoscilla Hemoglobin Gene (vgb)

In this study, the use of recombinant oxygen uptake system on production of ATPproduction, oxygen uptake and cell mass was investigated. Citrobacter freundii and their vgb recombinants were used. Citrobacter freundii, a broad host range plasmids Cf[pMK 79] and Cf[pUC 8:15] was used. Best ATP production by Cf[pMK 79] (0.17 mg/g). Cf[pMK 79] strain the level of oxygene uptake was higher than Cf[pUC 8:15].Whereas Cf[pMK 79] the OD600 values have been falling at the end of 48 hours.

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Zeng, A., Ross, A. and Deckwer, W., 1990. A method to estimate the efficiency of oxidative phosphorylation and biomass yield from ATP of a facultative anaerobe in continuous culture. Biotechnology and Bioengineering, 36, 965‐ 969.
Liu, H., Jiang, Y., Luo, Y. and Jiang, W., 2006. A simple and rapid determination of ATP, ADP and AMP concentrations in pericarp tissue of litchi fruit by high performance liquid chromatography. Food Technology and Biotechnology, 44(4), 531‐ 534.
Ivanova, E.P., Alexeeva, Y.V., Pham, D.K., Wright, J.P. and Nicolau, D.V., 2006. ATP level variations in heterotrophic bacteria during attachment on hydrophilic and hydrophobic surfaces. International Journal of Microbiology, 9, 37‐46.
Velten, S., Hammes, F., Boller, M. and Egli, T., 2007. Rapid and direct estimation of active biomass on granular activated carbon through adenosine tri‐phosphate (ATP) determination. Water Research, 41, 1973‐1983.
O’Mahony, K., Freitag, R., Hilbrig, F., Müller, P. and Schumacher, I., 2005. Proposal for a better integration of bacterial lysis into the production of plasmid DNA at large scale. Journal of Biotechnology, 119, 118‐132.
Wang, J., Chang, S., Chen, Y. and Luh, K., 2000. Comparison of antimicrobial susceptibility of Citrobacter freundii isolates in two different time periods. Journal of Microbiology, Immunology and Infection, 33, 258‐262.
Borenshtein, D. and Schauer, D.B., 2006. The genus Citrobacter. Prokaryotes, 6, 90‐98.
Lozano‐Leon, A, Iglesias‐Canle, J, Iglesias‐Garcia, J, Larino‐Noia, J. and Dominguez‐Muñoz, E., 2011. Citrobacter freundii infection after acute necrotizing pancreatitis in a patient with a pancreatic pseudocyst: a case report. Journal of Medical Case Reports, 5, 51‐54.
Erenler, S.O., Gencer, S., Geckil, H., Stark, B.C. and Webster, D.A., 2004. Cloning and Expression of the Vitreoscilla hemoglobin gene in Enterobacter aerogenes: effect on cell growth and oxygen uptake. Applied Biochemistry and Microbiology, 40(3), 241‐248.
Stark, B.C., Dikshit, K.L. and Pagilla, K.R., 2011. Recent advances in understanding the structure, function, and biotechnological usefulness of the hemoglobin from the bacterium Vitreoscilla. Biotechnology Letters, 33, 1705‐1714.
Geckil, H., Stark, B.C. and Webster, D.A., 2001. Genetically engineered Vitreoscilla hemoglobin gene. Journal of Biotechnology, 85, 5‐66.
Stark, B.C., Dikshit, K.L. and Pagilla, K.R., 2012. The Biochemistry of Vitreoscilla hemoglobin. Computational and Structural Biotechnology Journal, 3 (4), 1‐8.