Pınar NARTOP, Duygu DOLAPDERE, Gamze ARSLAN, Damla AKIN

Tohumların Yeşil Sterilizasyonu: Peganum harmala ve Rumex acetosella Tohumlarının Yüzeysel Sterilizasyonunda Biyosentetik Gümüş Nanopartiküllerin Kullanılması

Nanopartiküller teknolojinin birçok alanında sıklıkla kullanılmaktadırlar. Diğer metal nanopartiküller içerisinde en çok kullanılanlar gümüş nanopartiküllerdir. Kendilerine has özellikleri ortaya çıktıkça nanobiyoteknoloji alanındaki kullanımları da hızla artış göstermektedir. Gümüş nanopartiküllerin sentezinde farklı yöntemler kullanılmaktadır. Yeşil sentez (biyosentez) yöntemi gümüş nanopartikül elde edilmesindeki en ucuz ve pratik yoldur. Bu çalışmada, biyosentetik gümüş nanopartiküller biyoindirgeyici (kızılçam sulu ekstresi) kullanılarak sentezlenmiş ve iki tıbbi bitkiye (Perganum harmala ve Rumex acetosella) ait tohumların yeşil sterilizasyonunda kullanılmışlardır. Biyosentetik gümüş nanopartiküllerin yüzeysel sterilizasyon ve çimlenme yüzdeleri üzerine etkileri incelenmiştir. P. harmala thumlarının sterilizsyon yüzdeleri 20 ve 30 dakikalık uygulamalarda sırasıyla %84 ve %89.33 olarak belirlenmiştir. R. acetosella tohumlarında ise her iki uygulamada da yüzeysel sterilizasyon yüzdeleri %100’dür. Bu sonuçlar istatistiksel olarak kontrol grubundan farklı bulunmamışlardır (p>0.05). P. harmala tohumlarının çimlenme yüzdeleri denemelerden belirgin şekilde etkilenmişlerdir; 30 dakika uygulaması (%68) kontrol grubuna (%48) göre tohum çimlenmesini arttırmıştır. Ancak, R. acetosella tohumları biyosentetik gümüş nanopartikül uygulamalarından belirgin şekilde etkilenmemiştir. Bu sonuçlar bitki tohumlarının yüzeysel sterlizasyonunda biyosentetik gümüş nanopartiküllerin kimyasal sterilantlar yerine güvenle kullanılabileceğini göstermektedir.

Anahtar Kelimeler:

Biyosentetik gümüş nanopartikül, Yeşil sterilizasyon, Peganum harmala, Rumex acetosella, Tohum

Green Sterilization of Seeds: Use of Biosynthetic Silver Nanoparticles for Surface Sterilization of Peganum harmala and Rumex acetosella Seeds

Nanoparticles have frequently been used in many areas of technology. Silver nanoparticles are one of the most used nanoparticles amongst the other metal nanoparticles. Their use in nanobiotechnology is becoming more widespread as their unique properties become clearer. Different methods have been used to synthetize silver nanoparticles. Green synthesis (biosynthesis) method is the cheapest and the more practical way to obtain silver nanoparticles. In this study, biosynthetic silver nanoparticles were synthetized with the used of bioreducer (redpine water extracted) and used in the green sterilization of two medicinal plants’ seeds (Perganum harmala and Rumex acetosella). The effects of biosynthetic silver nanoparticles on surface sterilization and germination percentages were investigated. Sterilization percentages of P. harmala seeds were determined as 84% and 89.33%, when biosynthetic silver nanoparticle colloidal solution was applied for 20 and 30 mins, respectively. R. acetosella seeds’ surface sterilization percentages were 100% in both applications. These results were not found statistically significant than control group (p>0.05). Germination percentages of P. harmala seeds were significantly affected by the applications; 30 mins application (68%) enhanced seed germination when compared to control group (48%). However, R. acetocella seeds were not significantly affected by biosynthetic silver nanoparticle applications. These results showed that biosynthetic silver nanoparticles can be used safely instead of chemical sterilants in surface sterilization of plant seeds.

Keywords:

Biosynthetic silver nanoparticle, Green sterilization, Perganum harmala, Rumex acetosella, Seed,

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