Stachys tmolea Boiss. in Metanol ve Aseton Ekstraktlarının Toplam Fenolik İçeriği, Antioksidan Özellikleri ve Element Konsantrasyonları

Bu çalışmada Stachys tmolea Boiss. farklı çözücü ekstraktlarının DPPH radikal süpürücü ve toplam antioksidan aktivite ile fenolik ve element konsantrasyonları belirlenmiştir. Bitkinin metanol özütünün toplam fenolik madde içeriği 68.91±1.4 mg GAE/g ekstre olarak tespit edilmiştir. % DPPH radikal inhibisyonu 64.79±1.98 ve toplam antioksidan aktivitesi 2.28±0.21 mmol Trolox eşdeğeri/L dir. Aseton özütü 27,26±1,2 mg GAE/g ekstre toplam fenolik madde içerir. % DPPH radikali inhibisyonu 50.33±2.05 ve toplam antioksidan aktivitesi 0.57±0.04 mmol Trolox eşdeğeri/L dir. Metanol ekstraktının sentetik bir antioksidan olan bütillenmiş hidroksi anizole (toplam fenolik madde konsantrasyonu 82.33±2.1 mg GAE/g) yakın özelliklere sahip olduğu görülmektedir. Türün ayrıca süperoksit dismutaz ve katalaz enziminin yapısındfa bulunan Cu, Mn, Fe gibi elementleri de içerdiği belirlendi. Sonuç olarak, Stachys tmolea'nın metanolik özütü, radikal süpürücü ve antioksidatif özellikler sergiler. Türün içerdiği önemli elementler nedeniyle fitoterapi çalışmalarında değerlendirilebilecek bir bitki olduğu düşünülmektedir.

Anahtar Kelimeler:

Stachys tmolea, TAS, DPPH, Mineral

Total Phenolic Content, Antioxidant Properties and Element Concentrations of Methanol and Acetone Extracts of Stachys tmolea Boiss.

DPPH radical scavenging, total antioxidant status, phenolic and element concentrations of the different solvent extracts of Stachys tmolea Boiss. were determined in the study. It was found that, the methanol extract of the plant contains total phenolic substance is 68.91±1.4 mg Gallic acid equivalant/g extract. % DPPH radical inhibition was 64.79±1.98 and total antioxidant status was 2.28±0.21 mmol Trolox Equiv./L. The acetone extract contains total phenolic substance (27.26±1.2 mg GAE/g extract). % DPPH radical was 50.33±2.05, and total antioxidant status was 0.57±0.04 mmol Trolox Equiv./L. It is seen that, the methanol extract has properties close to the synthetic antioxidant butylated hydroxy anisole (total phenolic substance concentration is 82.33±2.1 mg GAE/g). It is determined that the species also contains elements such as Cu, Mn, and Fe that participate in superoxide dismutase and catalase. In conclusion, the methanolic extract of Stachys tmolea exhibits radical scavenging and antioxidative properties. The species is thought to be a plant that can evaluate in phytotherapy studies due to the important elements it contains.

Keywords:

Stachys tmolea, TAS, DPPH, Mineral,

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