Kumarin Yüklü Lipozomların Morfolojik ve Yapısal Özelliklerinin Belirlenmesi Üzerine Biyofiziksel Araştırma

Bu araştırmanın esas amacı kumarinin lipit model membranları nasıl etkilediğini incelemektir. Bu nedenle, zwitteriyonik lipit olarak dimiristoil fosfatidilkolin (DMPC) kullanılarak lipozom membranlar oluşturulmuştur. Kumarinin lipit membranların paketleme düzeni, akışkanlığı, hidrasyon durumu ve morfolojisi üzerine etkisi, özellikle mikroskobik (alan emisyonlu taramalı elektron mikroskobu (FE-SEM)) ve spektroskopik (zayıflatılmış toplam yansıma Fourier dönüşüm infrared (ATR-FTIR) spektroskopisi) teknikleri ile incelenmiştir. FE-SEM görüntüleri ve analizleri ile elde edilen sonuçlar dikkate alındığında, kumarinsiz ve kumarinli lipozomlar görünüm olarak düzgün yapılara ve küresel şekillere sahiptir. Bununla birlikte, kumarin yüklü lipozomların, yüklenmemiş lipozomların ortalama çapı ile karşılaştırıldığında boyutunda bir artış gözlenmiştir. ATR-FTIR analizleri göz önüne alındığında, DMPC lipitinin hidrofobik ve hidrofilik kısımlarına ait titreşim bantlarının incelenmesi, kumarinin sistemin düzenini azaltarak ve akışkanlığını artırarak ve zwitteriyonik lipit DMPC'nin arayüzey ve baş grup bölgeleri ile hidrojen bağı yaparak DMPC lipozomlarının fiziksel özelliklerini değiştirdiğini ortaya koymaktadır. Sonuç olarak, biyolojik olarak aktif bileşiklerin model membranlar ile etkileşimleri üzerine daha fazla biyofiziksel çalışmaların gerçekleştirilmesi, ilaç keşfi ve formülasyonlarında bu bileşiklerin moleküler etki mekanizmalarının belirlenmesinde önemli rol oynamaktadır.

Anahtar Kelimeler:

Kumarin, Lipozomlar, Fosfolipitler, ATR-FTIR, FE-SEM

A Biophysical Research on the Determination of Morphological and Structural Properties of Coumarin-Loaded Liposomes

The major goal of this research was to examine how coumarin affects lipid model membranes. For this reason, liposome membranes were formed using dimyristoyl phosphatidylcholine (DMPC) as zwitterionic lipid. The influence of coumarin on the morphology, packing order, fluidity, and hydration state of lipid membranes was specifically investigated by means of microscopic (field emission scanning electron microscopy (FE-SEM)) and spectroscopic (attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy) techniques. Taken into account the results obtained with FE-SEM images and analysis, liposomes without and with coumarin have uniform structures and spherical shapes in appearance. However, coumarin-loaded liposomes are observed with an increase in size when compared to a mean diameter of unloaded-liposomes. Considering ATR-FTIR analysis, the investigation of the vibrational bands which belong to the hydrophobic and hydrophilic parts of DMPC lipid reveals that coumarin alters the physical features of the DMPC liposomes by decreasing the order and increasing the fluidity of the system and making hydrogen bonding with the interfacial and headgroup regions of zwitterionic lipid DMPC. Finally, performing more biophysical studies on the interactions of biologically active compounds with model membranes plays an important role in determining the molecular action mechanisms of these compounds in drug discovery and formulations.

Keywords:

Coumarin, Liposomes, Phospholipids, ATR-FTIR, FE-SEM,

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