Sevgi ULUKÜTÜK, İbrahim Hakkı CİĞERCİ

Eisenia hortensis Sölomositlerinde Bentazone ve Chloridazon Herbisitlerinin Genotoksisitesinin Değerlendirilmesi

Bu çalışmada, Bentazone ve Chloridazon herbisitlerinin Eisenia hortensis türleri üzerindeki genotoksik etkileri araştırılmıştır. E. hortensis türleri Afyonkarahisar'daki doğal yaşam alanlarından toplanmıştır. Solucan sölomositlerinde genotoksisiteyi değerlendirmek için Comet ve Mikronükleus testi yapıldı. Bentazone herbisitlerin LD50 değeri Chloridazon için sırasıyla 236 ppm ve 76.6 ppm olarak kaydedildi. Daha sonra LD50/2, LD50 and 2XLD50 konsantrasyonlarında Bentazon ve Chloridazon herbisitleri E. hortensis'e 48 saat süreyle uygulandı. Her iki herbisitte de DNA'da konsantrasyona bağlı artış ve kromozomal hasar gözlendi (P

Genotoxic Evaluation of Bentazone and Chloridazon Herbicides in Eisenia hortensis Coelomocytes

In current study, genotoxic effects of Bentazone and Chloridazon herbicides on Eisenia hortensis specieswere investigated. The species of E. hortensis were collected from the natural habitats inAfyonkarahisar. Comet assay and Micronucleus test was conducted to evaluate the genotoxicity inearthworm coelomocytes. The LD50 value of Bentazone herbicides was noted as 236 ppm and 76.6 ppmfor Chloridazon, respectively. Then LD50/2, LD50 and 2XLD50 concentrations of Bentazone andChloridazon herbicides were applied to E. hortensis for 48 h. Concentration dependent increase in DNAand chromosomal damage was observed (P < 0.05) by both herbicides. Highest DNA damage andmicronucleus formation were noticed at highest doses compared to other concentrations and controlgroup. It was concluded that Bentazone and Chloridazon induced DNA damage and chromosomalaberrations failure in E. hortensis earthworms.

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