Sahra HOSSEINALIZADEH, Serap AÇIKGÖZ, Ömer ERİNCİK

Aydın İlinde Pamukta Rhizoctonia solani İzolatlarında Mikoviral Çift Sarmallı (ds) RNA'nın Araştırılması

Rhizoctoniasolani’ninpatojenikizolatlarının, farklı familyalardan birçok bitki türünde hastalığa neden olduğu bilinmektedir. Bu patojen, pamukta çökertene ve kök çürüklüğüne neden olan fungal patojenlerin en önemlilerinden biridir. Günümüzde hastalığa karşı kullanılan mücadele yöntemleri hastalık nedeniyle oluşan verim kayıplarının önlenmesinde yeterli olmamaktadır. Geçmişte, farklı ülkelerden R. solaniizolatları üzerinde yapılan çalışmalarda mycoviraldsRNA’lerin varlığı belirlenmiştir. Bu çalışmanın amacı; Aydın ilinde pamuklardan toplanan R. solaniizolatlarınınmikoviraldsRNA içeriklerinin araştırılmasıdır. Çalışma kapsamında, zayıf çökerten belirtileri gösteren pamuk fidelerinden elde edilmiş toplam 64 adet R. solaniizolatıdsRNA analizine tabi tutulmuştur. Altı R. solaniizolatında 12-18 kb büyüklüğünde dsRNAelektroforetikprofiller saptanmıştır.dsRNA pozitif 6 izolatınvirülensliğidsRNA-negatif bir izolat ile elma testinde karşılaştırılmıştır. İki dsRNA pozitif R. solani izolat elma üzerinde inokulasyon noktasında küçük lezyonlar oluşturmuştur ve bu durumun hipovirülenslik ile bir ilişkisi olabileceği düşünülmüştür.

Anahtar Kelimeler:

mikovirüs, Pamuk, Çökerten, Rhizoctonia solani, Hipovirülenslik

Investigation of Mycoviral double-stranded (ds) RNA in Rhizoctonia solaniIsolates from Cotton in Aydin Province/Turkey

Pathogenic forms of Rhizoctonia solani are known to attack many species of higher plants from different families. It is one of the causal agent of the damping-off and root rot of cotton. Thecurrentdamping-offmanagementpracticesare not satisfactoryforpreventingyieldlosses in cottonproduction. Hypovirulence, causedbyparticularmycoviruse species, is used in the management of certain plant diseases. The existence of mycoviral dsRNAs in the isolates of R. solani has been previously determined in various countries. ThisstudyaimedtoinvestigatedsRNAcontent in thecottonisolates of R. solanicollectedfromAydin Province. In total, 64 R. solani isolates collected from cotton seedlings exhibiting light damping-off symptoms were subjected to dsRNA analysis. The dsRNA electrophoretic pattern with the size of 12-18 kb were detected in six R. solani isolates. The virulence of these six isolates were compared with a dsRNA-free isolate in apple assay. Two R. solani isolates having dsRNA content caused smaller lesions in the inoculation point on apple fruit, which may be the indication of hypovirulence.

Keywords:

mycovirus, cotton, Damping off, Rhizoctonia solani, Hypovirulence,

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