Yeme Katılan Esansiyel Kekik Yağının Broylerlerde Plazma Lipid Konsantrasyonları Üzerine Etkisi

ÖZET Bu araştırma, etlik piliç yemlerine farklı düzeylerde esansiyel kekik yağı (Oreganum vulgare) ilave edilmesinin plazma total kolesterol, plazma HDL kolesterol ve plazma trigliserit üzerine etkilerini belirlemek amacıyla yapılmıştır. Araştırmada; 450 adet Ross 308 erkek broyler civciv deneme materyali olarak kullanılmıştır. Biri kontrol, ikisi deneme olmak üzere üç grup oluşturulmuştur. Her bir grup 15 civcivin bulunduğu 10 tekrar grubuna ayrılmıştır. Kontrol grubundaki civcivlere başlangıç (0-21 gün) ve büyütme (21-42 gün) dönemlerinde mısır - soya ağırlıklı temel rasyon yedirilmiştir. Deneme gruplarında ise temel rasyona birinci deneme grubunda 150 ppm esansiyel kekik yağı, ikinci deneme grubunda 300 ppm esansiyel kekik yağı ilave edilmiştir. 42 gün süren deneme periyodu boyunca yem ve su ad libitum olarak verilmiştir. Denemenin 21. ve 42. günlerinde V. subcutanea ulnaris'ten kan örnekleri alınarak, plazma total kolesterol, plazma HDL kolesterol ve plazma trigliserit analizleri ticari kitler kullanılarak spektrofotometrik yöntemle yapılmıştır. Denemenin 21. ve 42. gününde alınan kan örneklerinde, plazma total kolesterol düzeylerinde gruplar arasında istatistik önemde bir farklılık gözlenmemiştir. Çalışmanın 21. ve 42. gününde alınan kan örneklerinde 300 ppm kekik yağı ilave edilen grupta plazma HDL kolesterol düzeylerinin diğer gruplara göre önemli derecede düşük (P<0,05) olduğu belirlenmiştir. Plazma trigliserit düzeyleri bakımından gruplar arasında istatistiksel açıdan farklılık saptanmamıştır. Anahtar Kelimeler: Esans yağlar, broyler, kolesterol ABSTRACT EFFECT OF OREGANO ESSENTIAL OIL SUPPLEMENTATION ON PLASMA LIPID CONCENTRATIONS IN BROILERS The present experiment was conducted to describe the effects of dietary supplementation of oregano essential oil at different levels, on plasma concentrations of total and HDL cholesterol and triglycerides in broilers. A total 450 Ross 308 male chicks were used in the experiment. There were 3 dietary treatments, each consisting of ten replicates. A replicate was a cage with 15 birds so that each treatment had 150 animals. The control group was fed maise-soybean based diet while the other groups were fed diets supplemented with oregano essential oil at 150 and 300 ppm levels. The experiment lasted for 42 days. Feed and water were provided for ad libitum consumption. At 21 and 42 d of age, blood samples were collected from V. subcutanea ulnaris. Plasma concentrations of total and HDL cholesterol and triglycerides were measured spectrophotometrically by using commercial kits. There was no significant effect on plasma total cholesterol. At 21 and 42 days of age, plasma HDL cholesterol concentrations were significantly lower (P<0.05) in the birds given 300 ppm oregano essential oil when compared with the 150 ppm oregano essential oil group. Plasma triglyceride concentrations were not changed by any dietary treatment. Key Words: Essential oils, broiler, cholesterol

Anahtar Kelimeler:

Esans yağlar, broyler, kolesterol

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The present experiment was conducted to describe the effects of dietary supplementation of oregano essential oil at different levels, on plasma concentrations of total and HDL cholesterol and triglycerides in broilers. A total 450 Ross 308 male chicks were used in the experiment. There were 3 dietary treatments, each consisting of ten replicates. A replicate was a cage with 15 birds so that each treatment had 150 animals. The control group was fed maise-soybean Bu araştırma birinci yazarın "Yeme katılan esansiyel kekik yağının broylerlerde performans, bazı kan parametreleri ve sindirim enzimleri üzerine etkisi" isimli doktora tez çalışmasının bir kısmından özetlenmiştir. Yeme Katılan Esansiyel Kekik Yağının Broylerlerde Plazma Lipid Konsantrasyonları Üzerine Etkisi based diet while the other groups were fed diets supplemented with oregano essential oil at 150 and 300 ppm levels. The experiment lasted for 42 days. Feed and water were provided for ad libitum consumption. At 21 and 42 d of age, blood samples were collected from V. subcutanea ulnaris. Plasma concentrations of total and HDL cholesterol and triglycerides were measured spectrophotometrically by using commercial kits. There was no significant effect on plasma total cholesterol. At 21 and 42 days of age, plasma HDL cholesterol concentrations were significantly lower (P<0.05) in the birds given 300 ppm oregano essential oil when compared with the 150 ppm oregano essential oil group. Plasma triglyceride concentrations were not changed by any dietary treatment.

Keywords:

Essential oils, broiler, cholesterol,

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Alp, M., 1989. Tavuk beslenmesinde kullanılan yem maddelerinin ve karma yemlerin metabolize olabilir enerjilerinin hesaplanmasında yeni bir formül. Tavukçunun Sesi 1, 18-19.
AOAC, 1994. Official methods of analysis, association of official analytical chemists, Washington DC.
Babaoğlan, M., Tekeli, A., Filik, G., Kutlu, H R., 200 Etlik piliçlerin beslenmesinde büyüme uyarıcı olarak kullanılan farklı timol ve karvakrol kaynaklarının biyoetkinliklerinin Yeme Katılan Esansiyel Kekik Yağının Broylerlerde Plazma Lipid Konsantrasyonları Üzerine Etkisi 45 karşılaştırılması. V. Ulusal Hayvan Besleme Kongresi, Tekirdağ. Basmacıoğlu, H., Tokuşoğlu, Ö., Ergül, M., 2004. The effect of oregano and rosemary essential oils or alpha-tocopheryl acetate on performance and lipid oxidation of meat enriched with n-3 PUFA's in broilers. South African Journal Animal Science 34, 197-210.
Bassett, R., 2000. Oreganos positive impact on poultry production. World Poultry-Elsevier 16, 31Case, G . L . , He, L . , Mo, H., Elson, C . E . , 1995. Induction of geranyl pyrophosphate pyrophosphatase activity by cholesterolsuppressive isoprenoids. Lipids 30, 357-359.
Chao, S.C., Young, D.G., Oberg, C . J . , 2000. Screening for inhibitory activity of essential oils on selected bacteria, fungi and viruses. Journal of Essential Oil Research 12, 639-649.
Craig, W.J., 1999. Health-promoting properties of common herbs. American Journal of Clinical Nutrition 70, 491-499.
Dean, S.G., Ritchie, G., 1987. Antibacterial properties of plant essential oils. International Journal of Food Microbiology 5, 165-180
Dorman, H.J.D., Deans, S.G., 2000. Antimicrobial agents from plants antibacterial activity of plant volatile oils. Journal of Applied Microbiology 88, 308-316.
Düzgüneş, O., Kesici, T., Kavuncu, O., Gürbüz, F . , 19 Araştırma ve Deneme metotları. Ankara Üniversitesi Ziraat Fakültesi Yayınları 1021.
Eldeeb, M.A., Metwally, M.A., Galal, A.E., 2006. The impact of botanical extract, capsicum (capsicum frutescence), oil supplementation and their interactions on the productive performance of broiler chicks. X I I European Poultry Conference, Verona, Italy.
Elson, C . E . , 1995. Suppression of mevalonate pathway activities by dietary ısoprenoids: protective roles in cancer and cardiovascular disease. The Journal of Nutrition 125, 1666.
Giannenas, I . , Florou-Paneri, P., Papazahariadou, M., Christaki, E . , Botsoglou, N.A., Spais, A.B., 2003. Effect of dietary supplementation with oregano essential oil on performance of broilers after experimental infection with
Eimeria tenella. Archiv für Tierernahrung 57, 99Horton, G.M.J., Fennel, M.J., Parasad, B.M., 1991.
Effect of dietary garlic (Allium sativum) on performance, carcass composition and blood chemistry changes in broiler chickens. Canadian Journal of Animal Science 71, 939¬ 9 Lee, K.G., Shibamoto, T., 2002. Determination of antioxidant potential of volatile extracts isolated from various herbs and spices. Journal of Agricultural and Food Chemistry 50, 4947¬ 49
Lee, K.W., Everts, H., Kappert, H.J., Frehner, M., Losa, R., Beynen, A.C., 2003a. Effects of dietary essential oil components on growth performance, digestive enzymes and lipid metabolism in female broiler chickens. British Poultry Science 44, 450-457.
Lee, K.W., Everts, H., Kappert, H.J., Yeom, K.H., Beynen, A.C., 2003b. Dietary carvacrol lowers body weight gain but improves feed conversion in female broiler chickens. The Journal of
Applied Poultry Research 12, 394-399. Lopez-Bote, C.J., Gray, J.I., Gomaa, E.A., Flegal, C.J., 1998. Effect of dietary administration of oil extracts from rosemary and sage on lipid oxidation in broiler meat. British Poultry Science 39, 235-240.
National Research Council, 1994. Nutrient requirements of poultry, 9 t h rev. Ed. National
Academy Press, Washington, DC. Sivropoulou, A., Papanikolaou, E . , Nikolaou, C., Kokkini, S., Lanaras, T., Arsenakis, M., 19 Antimicrobial and cytotoxic activities of Origanum essential oils. Journal of Agricultural and Food Chemistry 44, 1202¬ 120
SPSS, 1999. SPSS for Windows. Base System User's
Guide, Release 10.1, SPSS Inc., Chicago IL, USA, 1999.
Yu, S.G., Abuirmeileh, N.M., Qureshi, A.A., Elson, C.E., 1994. Dietary p-ionone suppresses hepatic 3-hydroxy-3-methylglutaryl coenzyme a reductase activity. Journal of Agricultural and Food Chemistry 42, 1493-1496.