Kafes Kuşlarının Gaitalarında Candida Türlerinin Araştırılması

ÖZET Bu çalışmanın amacı, İstanbul'da kafes kuşlarında Candida cinsi mayaların baskın türlerini farklı kültür ve identifikasyon teknikleri ile karşılaştırmalı olarak belirlemektir. Bu amaçla, İstanbul'da çeşitli yetiştiricilerde, satıcılarda ve evlerde beslenen kafes kuşlarının taze dışkı örnekleri steril besiyerli svab yardımıyla kafes tabanından alınmış ve soğuk zincir altında laboratuvara getirilmiştir. Örneklerden kloramfenikol katkılı Sabouraud Glukoz Agar, kloramfenikol ve sikloheksimid katkılı Sabouraud Dekstroz Agar ve Chromogenic Candida Agar besiyerlerine ekimler yapılmıştır. Kandida şüpheli kolonilerin identifikasyonu için, API 20 C AUX sistem ile asimilasyon testleri, karbonhidrat fermentasyon testleri, üreaz, nitrat redüksiyonu ve sitrat kullanımı testleri yapılmıştır. Doksan yedi kafes kuşunun 13'ünde (%13,4) dışkıda maya saptanmıştır. Bunlardan 9'u (%69,23) Candida spp. olarak tanımlanmış olup 5'i Candida famata, 4'ü Candida albicans olarak identifiye edilmiştir. Anahtar Kelimeler: Candida, gaita, kafes kuşları, kandidiyaz, psittasin ABSTRACT INVESTIGATION OF CANDIDA SPECIES IN FECAL SAMPLES TAKEN FROM CAGE BIRDS The goal of this study was to isolate and identify of Candida spp. comparatively by different isolation and identification methods and to determine the predominant species in cage birds of Istanbul. For this purpose, fresh stool samples of cage birds, raised in various pet shops and houses, were collected from the bottom of the cages with sterile swabs, and transported to the laboratory in cold chain. Samples were cultured onto Sabouraud Glucose Agar with chloramphenicol, Sabouraud Dextrose Agar with cycloheximide and chloramphenicol, and Chromogenic Candida Agar media. API 20 C AUX system, carbonhydrate fermentation tests, urease, nitrate reduction and citrate utilization tests were used for identification of pure cultures of suspected colonies of Candida. Yeasts were isolated from 13 (13.4%) of 97 cage bird stool samples. Nine (69.23%) of the isolates were identified as Candida spp.; Four of them were identified as Candida albicans while five of them were Candida famata. Key Words: Cage birds, Candida, candidiasis, psittacine, stool sample

Anahtar Kelimeler:

Candida, gaita, kafes kuşları, kandidiyaz, psittasin

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The goal of this study was to isolate and identify of Candida spp. comparatively by different isolation and identification methods and to determine the predominant species in cage birds of Istanbul. For this purpose, fresh stool samples of cage birds, raised in various pet shops and houses, were collected from the bottom of the cages with sterile swabs, and transported to the laboratory in cold chain. Samples were cultured onto Sabouraud Glucose Agar with chloramphenicol, Sabouraud Dextrose Agar with cycloheximide and chloramphenicol, and Chromogenic Candida Agar media. API 20 C AUX system, carbonhydrate fermentation tests, urease, nitrate reduction and citrate utilization tests were used for identification of pure cultures of suspected colonies of Candida. Yeasts were isolated from 13 (13.4%) of 97 cage bird stool samples. Nine (69.23%) of the isolates were identified as Candida spp.; Four of them were identified as Candida albicans while five of them were Candida famata.

Keywords:

Cage birds, Candida, candidiasis, psittacine, stool sample,

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