Özgür Genç ŞEN, Hacer Deniz ARISU, Gürcan ESKİTAŞÇIOĞLU, Evrim Eligüzeloğlu DALKILIÇ, Hüma ÖMÜRLÜ

HPLC analysis of monomers eluted from self-adhesive resin cements

AMAÇ: Bu çalışmanın amacı, Light Emitting Diode (LED) veya halojen ışık kaynaklarıyla sertleştirilmiş iki kendin-den bağlanabilen rezin simanın artık monomer salımını belirlemektir.GEREÇ VE YÖNTEM: Clearfil SA (grup A, n = 48) ve BisCem (grup B, n = 48) simanlar plastik kalıplara yerleştirildi. Her grup kendi içinde ikiye ayrıldı. Grup A1 ve B1'deki örnekler LED, grup A2 ve B2'deki örnekler halojen ışık kaynaklarıyla polimerize edildi. Distile suda tutulan örneklerden salınan trietilen glikol-dimetakrilat (TEGDMA) ve bisfenol A glisidilmetakrilat (Bis-GMA) bileşikleri, 1 saat ve 24 saat enkübasyon sonrasında yüksek performanslı likit kromotografisi (HPLC) ile incelendi. İstatistiksel değerlendirmeler için faktöriyel deneysel dizayn ve t tes-tleri kullanıldı.BULGULAR: Kendinden bağlanabilen rezin simanlar LED ışık kaynaklarıyla sertleştirildiğinde daha fazla Bis-GMA ve TEGDMA monomeri saldılar (p0.05). Clearfil SA siman BisCem'den daha fazla Bis-GMA saldı (p

Kendinden bağlanabilen rezin simanlardan salınan monomerlerin HPLC ile incelenmesi

OBJECTIVE: The aim of this study was to determine the residual monomer leaching from two self-adhesive resin cements polymerized with Light Emitting Diode (LED) or halogen light curing unit.MATERIALS AND METHOD: Clearfil SA (group A, n = 48) and BisCem (group B, n = 48) cements were inserted in plastic moulds. Each group was further divided into two subgroups. Specimens were light cured with LED light curing unit (LCU) in group A1 and group B1 and halogen LCU in group A2 and group B2 for 20 seconds. The following compounds released from the samples stored in distilled water were analyzed: triethylene glycol-dimethacrylate (TEGDMA) and bisphenol A glycidyl methacrylate (BisGMA). Analysis of substances was performed with the use of high performance liquid chromatography, after 1 hour and 24 hour incubation periods. Factorial experimental design and independent t-test was used for statistical analyses.RESULTS: Self-adhesive resin cements released more BisGMA and TEGDMA when they were polymerized with LED LCUs (p0.05). Clearfil SA cement released more Bis-GMA than BisCem (p

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