A Comparison of Thiol/Disulphide Levels of Workers in Operating Rooms and Emergency Healthcare Centers

Objective: Thiol/disulphide homeostasis has a crucial role for life. The aim of this study was to compare thiol/disulphide homeostasis in healthcare professionals work- ing in the operating room (Group 1) and healthcare professionals working in the emergency department (Group 2). Materials-Methods: This was a descriptive study. Fortynine operating room volun-teers (Group 1) and 49 emergency department volunteers (Group 2) were included in this study. Thiol and disulphide concentrations were measured using the novel auto-mated measurement method developed by Erel & Neşelioğlu in 2014. Result: The thiol/disulphide levels were compared in both groups; Native thiol in group 1 was 353,57 ± 49,86 μmol.L -1 , and it was 333,73 ± 37,38 μmol.L -1 (p = 0.028) in group 2. total thiol values were 384,71 ± 52,36 μmol.L−1 and 370,94 15,56 ± 5,36 μmol.L -1 (P =0,133), respectively in group 1 and 2. In group 1,disulphide levels were 15,56±5,36 μmol.L -1 , in group 2 it was 18,61 ± 7,71 μmol.L -1 (p = 0,025), disulphide/native thiol in group 1 was 4,47 ± 1 %, and in group 2 it was measured %, 5,73 ± 2,95% (p = 0.009). Disulphide/total thiol in the group 1 was 4,06 ± 1,32% and 5,03 ± 2,13% (p= 0,008) in group 2. Native thiol / total thiol levels were 90,23 ± 12,16% in group 1 and 89,93 ± 4,26% in group 2 (p = 0,871). Native thiol level in group 1 and high level of di-sulphide in group 2 indicate that the exposure to oxidative stress in the emergency de-partment is higher than in the operating room. Conclusion: Our results show that participants of emergency room group have statis-tically higher levels of disulphide than participants working in operating room which may suggest presence of oxidative stress related with workplace exposures.

Kaynakça

McCord JM. The evolution of free radicals and oxidative stress. Am J Med 2000; 108(8): 652-59.

Valko M, Leibfritz D, Moncol J, et al. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007; 39(1): 44-84.

Kumar S. Free Radicals and Antioxidants: Human and Food System. Pelagia Research Library Advances in Applied Science Research 2011; 2(1): 129-35.

Ulas T, Buyukhatipoglu H, Kirhan I, et al. The effect of day and night shifts on oxidative stress and anxiety symptoms of the nurses. Eur Rev Med Pharmacol Sci 2012; 16(5): 594-99. Sharifian A, Farahani S, Pasalar P, et al. Shift work as an oxida- tive stressor. Journal of Circadian Rhythms 2005; 3: 15.

Gromadzińska J, Peplonska B, Sobala W, et al. Relationship between intensity of night shift work and antioxidant status in blood of nurses. Int Arch Occup Environ Health. 2013; 86(8): 923-30.

Malekirad AA, Ranjbar A, Rahzani K et al. Oxidative stress in operating room personnel: occupational exposure to anesthetic gases Human & Experimental Toxicology 2005; 24: 597 - 601.

Lucio LMC, Braz MG, Nascimento JP, et al. Occupational hazards, DNA damage, and oxidative stress on exposure to waste anesthetic gases. Rev Bras Anestesiol 2018; 68(1): 33-41.

Erel O, Neselioglu S. A novel and automated assay for thiol/di- sulphide homeostasis. Clin Biochem 2014; 47(18): 326-32.

Comini MA. Measurement and meaning of cellular thiol: disufhide redox status. Free Radic Res 2016; 50(2): 246-71.

Chianeh YR, Prabhu K. Protein thiols as an indicator of oxidative stress. Archives Medical Review Journal 2014; 23(3): 443-456.

Ates I, Ozkayar N, Inan B, et al. Dynamic thiol/disulphide homeostasis in patients with newly diagnosed primary hypertension J Am Soc Hypertens 2016;10(2):159-66.

Ates I, Kaplan M, Yuksel M, et al. Determination of thiol/disul- phide homeostasis in type 1 diabetes mellitus and the factors associated with thiol oxidation. Endocrine 2016; 51(1): 47-51.

Çakıcı EK, Eroğlu FK, Yazılıtaş F, et al. Evaluation of the level of dynamic thiol/disulphide homeostasis of in adolescent patients with newly diagnosed primary hypertension. Pediatr Nephrol 2018; 33(5): 847-53.

Kundi H, Erel Ö, Balun A. Association of thiol/disulfide ratio with syntax score in patients with NSTEMI. Scand Cardiovasc J 2015; 49(2): 95-100.

Gumusyayla S, Vural G, Bektas H, et al. A novel oxidative stress marker in patients with Alzheimer’s disease: dynamic thiol– disulphide homeostasis. Acta Neuropsychiatr 2016; 4: 1-6.

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