A Comparison of Thiol/Disulphide Levels of Workers in Operating Rooms and Emergency Healthcare Centers

Objective: Thiol/disulphide homeostasis has a crucial role for life. The aim of this study was to compare thiol/disulphide homeostasis in healthcare professionals work- ing in the operating room (Group 1) and healthcare professionals working in the emergency department (Group 2). Materials-Methods: This was a descriptive study. Fortynine operating room volun-teers (Group 1) and 49 emergency department volunteers (Group 2) were included in this study. Thiol and disulphide concentrations were measured using the novel auto-mated measurement method developed by Erel & Neşelioğlu in 2014. Result: The thiol/disulphide levels were compared in both groups; Native thiol in group 1 was 353,57 ± 49,86 μmol.L -1 , and it was 333,73 ± 37,38 μmol.L -1 (p = 0.028) in group 2. total thiol values were 384,71 ± 52,36 μmol.L−1 and 370,94 15,56 ± 5,36 μmol.L -1 (P =0,133), respectively in group 1 and 2. In group 1,disulphide levels were 15,56±5,36 μmol.L -1 , in group 2 it was 18,61 ± 7,71 μmol.L -1 (p = 0,025), disulphide/native thiol in group 1 was 4,47 ± 1 %, and in group 2 it was measured %, 5,73 ± 2,95% (p = 0.009). Disulphide/total thiol in the group 1 was 4,06 ± 1,32% and 5,03 ± 2,13% (p= 0,008) in group 2. Native thiol / total thiol levels were 90,23 ± 12,16% in group 1 and 89,93 ± 4,26% in group 2 (p = 0,871). Native thiol level in group 1 and high level of di-sulphide in group 2 indicate that the exposure to oxidative stress in the emergency de-partment is higher than in the operating room. Conclusion: Our results show that participants of emergency room group have statis-tically higher levels of disulphide than participants working in operating room which may suggest presence of oxidative stress related with workplace exposures.

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McCord JM. The evolution of free radicals and oxidative stress. Am J Med 2000; 108(8): 652-59.

Valko M, Leibfritz D, Moncol J, et al. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007; 39(1): 44-84.

Kumar S. Free Radicals and Antioxidants: Human and Food System. Pelagia Research Library Advances in Applied Science Research 2011; 2(1): 129-35.

Ulas T, Buyukhatipoglu H, Kirhan I, et al. The effect of day and night shifts on oxidative stress and anxiety symptoms of the nurses. Eur Rev Med Pharmacol Sci 2012; 16(5): 594-99. Sharifian A, Farahani S, Pasalar P, et al. Shift work as an oxida- tive stressor. Journal of Circadian Rhythms 2005; 3: 15.

Gromadzińska J, Peplonska B, Sobala W, et al. Relationship between intensity of night shift work and antioxidant status in blood of nurses. Int Arch Occup Environ Health. 2013; 86(8): 923-30.

Malekirad AA, Ranjbar A, Rahzani K et al. Oxidative stress in operating room personnel: occupational exposure to anesthetic gases Human & Experimental Toxicology 2005; 24: 597 - 601.

Lucio LMC, Braz MG, Nascimento JP, et al. Occupational hazards, DNA damage, and oxidative stress on exposure to waste anesthetic gases. Rev Bras Anestesiol 2018; 68(1): 33-41.

Erel O, Neselioglu S. A novel and automated assay for thiol/di- sulphide homeostasis. Clin Biochem 2014; 47(18): 326-32.

Comini MA. Measurement and meaning of cellular thiol: disufhide redox status. Free Radic Res 2016; 50(2): 246-71.

Chianeh YR, Prabhu K. Protein thiols as an indicator of oxidative stress. Archives Medical Review Journal 2014; 23(3): 443-456.

Ates I, Ozkayar N, Inan B, et al. Dynamic thiol/disulphide homeostasis in patients with newly diagnosed primary hypertension J Am Soc Hypertens 2016;10(2):159-66.

Ates I, Kaplan M, Yuksel M, et al. Determination of thiol/disul- phide homeostasis in type 1 diabetes mellitus and the factors associated with thiol oxidation. Endocrine 2016; 51(1): 47-51.

Çakıcı EK, Eroğlu FK, Yazılıtaş F, et al. Evaluation of the level of dynamic thiol/disulphide homeostasis of in adolescent patients with newly diagnosed primary hypertension. Pediatr Nephrol 2018; 33(5): 847-53.

Kundi H, Erel Ö, Balun A. Association of thiol/disulfide ratio with syntax score in patients with NSTEMI. Scand Cardiovasc J 2015; 49(2): 95-100.

Gumusyayla S, Vural G, Bektas H, et al. A novel oxidative stress marker in patients with Alzheimer’s disease: dynamic thiol– disulphide homeostasis. Acta Neuropsychiatr 2016; 4: 1-6.