Behire Işıl DİDİNEN, Ayşegül KUBİLAY, Seçil METİN, Bülent BAŞ, Tom WIKLUND, Pınar YILDIRIM

Türkiye’deki Gökkuşağı Alabalığı(Oncorhynchus mykiss, Walbaum) Fryları’ndan Candida sp.’nin İlk Bildirimi

Bu çalışmada, Türkiye'de gökkuşağı alabalıklarında (Oncorhynchus mykiss) ilk defa Candida sp. izole edilmiştir. Hastalık, yavrularda (ortalama 0,2 gr) 2016 Şubat ayında 10°C su sıcaklığında gözlenmiştir. Hasta balıkların solungaçlarında maya hücrelerinin birikimine bağlı olarak beyaz renkli alanlar ve mikroskop altında sekonder lamellalarda yapışma gözlenmiştir. Böbrek, dalak ve solungaçlardan alınan mikrobiyolojik örnekler tryptik soya agar (TSA) ve trypton yeast extract salts (TYES) agar üzerine ekilmiş ve TSA 22° C ve TYES agar da 18° C'de 48 saat boyunca inkübe edilmiştir. Hasta balıklardan on üç izolat elde edilmiş ve izolatların tümünden Gram boyama yapılmıştır. Işık mikroskobu kullanılarak hücresel morfolojiye göre tüm izolatların maya oldukları saptanmıştır. İdentifikasyon, ITS (Internal Transcribed Spacer) bölgesinin sekans analizi ile gerçekleştirilmiş ve sekans verilerine göre D11 izolatının Candida'ya ait olduğu tespit edilmiştir. Maya enfeksiyonunun, alabalıkların erken gelişim evrelerinde (larva ve yavru) görülen gökkuşağı alabalığı fry sendromu (RTFS) hastalığının tedavisinde yoğun olarak antibiyotik kullanımına bağlı olarak ortaya çıktığı düşünülmektedir.

First Report of Candida sp. from Rainbow Trout (Oncorhynchus mykiss, Walbaum) Fry in Turkey

In this study, we isolated Candida sp. for the first time from fry of rainbow trout (Oncorhynchus mykiss) in Turkey.Candida species caused disease in fry (average 0.2 g) which was observed at 10°C water temperature in February 2016. Dueto yeast accumulation on gills, whitish areas was seen and fusion of secondary lamella in gills were observed undermicroscope. The microbiological samples of kidney, spleen and gills were streaked onto trypticase soy agar (TSA) andtryptone yeast extract salts (TYES) agar plates and incubated at 22°C in TSA and 18°C in TYES agar for 48 h. Thirteenisolates were obtained and Gram staining was performed. The all of the isolates were determined as yeast cell based oncellular morphology by using light microscopy. The identification was performed by ITS (Internal transcribed spacer) regionsequence analysis and sequence data demonstrated that representative isolate D11 belonged to genus Candida. The yeastinfection might have seen in early development stages (larvae and fry) due to the intensive antibiotic use against rainbowtrout fry syndrome (RTFS) disease.

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