İmidakloprid Uygulamasının Zebra Balığı (Danio rerio) İnce Bağırsak Dokusunda Oluşturduğu Histopatolojik Etkiler

Tarımda verimliliği artırmak için pestisit kullanımı gün geçtikçe artmaktadır. Tarımsal alanda kullanılan pestisitler, yağmurlar, yeraltı suları vb. yollarla sucul ekosisteme karışmakta ve sucul canlıların yaşamını tehdit etmektedir. Günümüzde en çok kullanılan pestisit gruplarından biri de neonikotinoidlerdir. Imidakloprid, bu grubun içinde en çok bilinenidir. Bu çalışmada imidaklopridin zebra balığı bağısak dokusunda yarattığı histopatolojik etkilerin gözlenmesi amaçlanmıştır. Balıklara 5 gün boyunca 9,5, 19 ve 38 mg/L’lik konsantrasyonlarda imidakloprid uygulanmıştır. Dokular ışık mikroskobunda incelendiğinde, villus morfolojisinde bozulma, epitel hücrelerinde fırçamsı kenar yapısında dejenerasyon, inflamasyona bağlı olarak Goblet hücrelerinde ve enterositlerde hiperplazi, villuslarda birleşme, lamina propriada genişleme ve dağılma, muscularis externa tabakasında kalınlaşma ve bu tabakadaki düz kas hücrelerinde hipertrofi gibi etkiler gözlenmiştir. En yüksek konsantrasyon grubuna ait örneklerde ince bağırsak dokularında displazi tespit edilmiştir.

Anahtar Kelimeler:

imidakloprid, ince bağırsak, histopatoloji, zebra balığı, teleost

Histopathological Evaluation of Zebrafish (Danio rerio) Intestinal Tissue After Imidacloprid Exposure

The use of pesticides has been increasing day by day in order to increase productivity in agriculture. Pesticides which used in the agricultural area mix into the aquatic ecosystem through rains and groundwater etc. and it threatens the life of aquatic organisms. Nowadays, one of the most used pesticide group is neonicotinoids. Imidacloprid is the most well known in this group. In this study, it was aimed to observe the histopathological effects of imidacloprid in zebrafish intestinal tissue. Adult zebrafish individuals were administered imidacloprid in concentrations of 9.5, 19 and 38 mg/L for 5 days. When tissues are examined under light microscopy, deterioration of villi morphology, degeneration in brush border structure of epithelial cells, hyperplasia in Goblet cells and enterocytes due to inflammation, enlargement and disintegration in lamina propria were detected. In addition, hypertrophy of the smooth muscle cells and thickening in the muscularis externa layer were observed. Dysplasia was detected in the small intestine tissues in samples belonging to the highest concentration group.

Keywords:

intestine, imidacloprid, histopathology, zebrafish, teleost,

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