Ratlarda Trinitrobenzensulfonik Asit ile Oluşturulan Deneysel Kolitte Kısa Zincirli Yağ Asitlerinin Epitel Onarımına Etkisi

Amaç: Bu araştırmanın amacı, trinitrobenzensulfonikasid TNBS ile deneysel kolit oluşturulan ratları kullanarak kısa zincirli yağ asitlerinin ülseratif kolit tedavisindeki yerini tartışmaktır. Yöntem: Bu araştırmada, deneysel araştırma yöntemi kullanılmıştır. Ortalama ağırlıkları 150±30 gr. arasında değişen 30 dişi Wistar cinsi rat çalışma grubunu oluşturmuştur. Ratlar randomize olarak 10’ar adetlik üç gruba ayrılmıştır. Gruplar, iki grup deney, bir grup kontrol grubu olmak üzere yine randomize olarak atanmıştır. Birinci deney grubuna 3. günden itibaren günde iki kez intrakolonik olarak KZYA, ikinci deney grubuna ilk günden itibaren günde iki kez KZYA olarak uygulanmıştır. Kontrol grubuna ise 3. günden itibaren günde iki kez serum fizyolojik uygulanmıştır. 6. günde çalışma sonlandırılarak ratlar dekapite edilmiştir. Kolon mukozasının makroskopik ve mikroskobik değerlendirmesiyle MPO aktivitesi değerlendirmesi yapılmıştır. Bulgular: 1.-2., 2.-3., 1.-3. grupların kolon makroskopileri arasında istatistiksel olarak anlamlı fark bulunmuştur p

The Effect of Short-Chain Fatty Acids On Epithelial Repair In Experimental Colitis Created With Trinitrobenzenesulfonic Acid In Rats

Objectives: The purpose of this study is to discuss short-chain fatty acids’ SCFA role in ulcerative colitis UC treatment by using rats which have experimental colitis created with trinitrobenzenesulfonic acid TNBS . Method: In this study, the experimental research method was adopted. The study group consisted of 30 female Wistar rats which have 150±30 gr. average weights, Rats were randomly divided into 3 groups with 10 rats in each group. The groups were randomly assigned as two experimental groups and one control group. The first experimental group was administered SCFA intracolonically 2 times a day from the 3rd day, and the second experimental group was administered SCFA 2 times a day from the first day. The control group was given saline 2 times a day from the 3rd day. On day 6, the rats were decapitated. MPO activity evaluation and macroscopic and microscopic evaluation of the colon mucosa was done. Results: There was a statistically significant difference between the colon macroscopy results of the groups 1-2, 2-3, 1-3 p

Keywords:

Short-chain fatty acids. ulcerative colitis, trinitrobenzenesulfonic acid,

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