In Utero Etanol Uygulamasının Sıçan Testis Morfolojisi Üzerine Etkileri

Amaç: Etanol, erkeklerde testislerde atrofiye, germ hücrelerinde kayba, sperm üretiminde azalmaya, seminifer tübül çaplarında azalmaya ve testis ağırlığında düşüşe neden olur. Gebelik döneminde etanol uygulaması hem dişilerde hem de erkeklerde reprodüktif fonksiyonları bozarak fetuste teratojenik etki göstermektedir. Bu çalışmanın amacı in utero etanol uygulanan sıçanların testisindeki değişimleri incelemektir. Yöntem: Bu çalışmada 2 deney grubu kullanılmıştır: 1 Kontrol grubu. 2 Alkol grubu. Alkol grubundakilere gestasyonel 14.günden itibaren doğuma kadar %30 etanol içeren sıvı verilmiştir. Deney ve kontrol grubundaki anne sıçanlardan doğan erkek yavrulardan 0., 15., 30. ve 90. günlük dönemlerde testis örnekleri alınmış, rutin parafin takibi uygulanmış ve kesitlere morfolojik inceleme için Masson’un üçlü boyaması uygulanmıştır. Bulgu: Alkol neonatal grubunda bazı seminifer tübüllerde düzensizlikler gözlenirken zamana bağlı olarak seminifer tübüllerde dejenerasyon ve spermatogenik hücre serilerinde azalma gözlenmiştir. Alkol neonatal ve postnatal 15. gün grubunda mezenkimal bağ dokusunun fazla olduğu, postnatal 30 gün ve 90 gün grubunda da interstisyel bağ dokunun artmış olduğu görülmüştür. Sonuç: Sonuç olarak, gestasyonel dönemde alınan alkol, erkek yavrularda seminifer tübüllerde spermatogenik hücre serilerinde azalmaya neden olmaktadır. Buna bağlı olarak gebelik döneminde alınan alkol, erkeklerde testiste atrofiye ve spermatogenik serideki hücre sayısını azaltarak infertiliteye sebep olabilir.

Alterations of Testicular Morphology In Rats Exposed To In Utero Ethanol

Aim: Ethanol causes testicular atrophy, loss in germ cells,decrease in sperm production, decrease in the radius of seminiferous tubule lines and decrease in the testicular weight. Ethanol use in pregnancy also causes teratogenic effects in fetus by degenerating reproductive functions both in males and females. The aim of this study is to examine the testicular changes in rats which are applied ethanol in utero. Method: 2 experimental groups were used: 1 Control group 2 Alcohol group. Alcohol group was given liquid containing 30% ethanol beginning from the 14th gestational day until birth. Testicular samples were obtained from 0, 15, 30 and 90-day-old offsprings of mother rats in experiment and control groups. Sections were administered routine paraffin method and stained with Masson’s trichrome for morphologic examination. Result: As in alcohol neonatal group some disorders in seminiferous tubules were observed, a degeneration in seminiferous tubules and decrease in spermatogenic cell series were observed related with the time. In alcohol neonatal and postnatal 15 days group mesenchymal tissue, in postnatal 30 days and 90 days groups the interstitiel tissue were observed to be more. Conclusion: As a result in utero ethanol administration cause severe degeneration in the spermatogenic cell series in the seminiferous tubules of the male offsprings. Related to this, in utero ethanol causes infertility by decreasing the number of cells in spermatogenic series and testicular atrophy in males.

Keywords:

Ethanol, diffrentiation, infertility morphology, testis,

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