Bertan KESİM, Birsen KORKMAZ

Konsantre büyüme faktörünün hücre ve dokular üzerindeki etkileri ve rejeneratif tedavide uygulama alanları

Konsantre büyüme faktörü (KBF) karmaşık üç boyutlu fibrin ağ yapısı içerisinde trombositler, lökositler, kök hücreler ve sitokinler içeren otolog bir trombosit konsantresidir. KBF'nin ekstraksiyonu için kullanılan özel santrifüj cihazı, değiştirilmiş hız oranını kullanmaktadır. Üretimleri sırasındaki santrifüj hızlarının farklı olmasının sonucu olarak içeriğindeki fibrin bloklarının daha geniş, daha yoğun ve daha fazla miktarda büyüme faktörü içerdiği bildirilmiştir. Ayrıca KBF’nin yüksek gerilme dayanıklılığı ve viskozitesi, büyüme faktörlerini proteolizisden daha iyi korumakta ve büyüme faktörlerinin salınım süresini uzatmaktadır. Böylece KBF’nin entegre bir büyüme faktörü rezervuarına sahip güçlü bir biyomateryale dönüştüğü ileri sürülmektedir. Tüm bu özellikleriyle KBF’nin rejeneratif potansiyel ve klinik kullanım açısından daha iyi özelliklere sahip olması beklenmektedir. Çoğu çalışmada KBF’nin doğal bir iskelet ve rejeneratif tedavilerde büyüme faktörlerinin rezervuarı olarak faydalı etkiler oluşturabileceği bildirilmiştir. Ayrıca KBF’nin belirli konsantrasyonlar altında doza bağlı şekilde olumlu etkileri gösterilmiş ve KBF’yi diğer trombosit konsantreleri ile karşılaştıran çalışmalarda farklı sonuçlar bildirilmiştir. Bu derlemenin amacı KBF’nin in vitro ve in vivo biyolojik etkileri ile etkinliğine yönelik yapılan çalışmaları özetlemek ve bu çalışmaların sonuçlarını analiz ederek değerlendirmektir.

Effects of concentrated growth factor on cells and tissues and applications in regenerative therapy

Concentrated growth factor (CGF) is an autologous platelet concentrate that contains of platelets, leukocytes, stem cells and cytokines with in a complex three - dimensional fibrin network. Special centrifuge used for extraction of CGF, it is utilizes altered speed rate. As a result of different centrifuge speeds permit the isolation of much larger, denser and richer growth factors in matrix from CGF. In addition, the high tensile strength and viscosity of CGF protects from proteolysis and prolongs release time of growth factors. Thus, it is suggested that CGF is a strong biomaterial with an integrated growth factor reservoir. With all these features, CGF is expected to have better properties in terms of regenerative potential and clinical use. In addition, the positive effects of CGF were in a dose dependent manner under certain concentrations and different results were reported in studies comparing with other platelet concentrates. The aim of this review is to summarize these studies which in vitro and in vivo biological effects and effectiveness of CGF and to analyze and evaluate the results of these studies.

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