Zeytin (Olea europaea L.) Genotiplerinin DNA Markörleri Yardımı ile Karakterizasyonu
Bu çalışmada ulusal zeytin arazi gen bankasındaki 96 genotip DNA’ya dayalı yöntemler olan RAPD, AFLP ve
SSR markör teknikleri uygulanarak moleküler düzeyde tanımlanmıştır. RAPD markör analizinde 52 primerden
215 polimorfik bant, AFLP markör analizinde 26 primerden 919 polimorfik bant ve SSR markör analizinde ise 14
primerden 62 polimorfik bant elde edilmiştir. Her teknikten elde edilen veriler ile genetik uzaklık matrisi ve
dendrogram oluşturulmuştur. Ayrıca üç tekniğin verilerinin birleştirilmesi sonucunda toplam 1196 adet
polimorfik bant değerlendirilerek dendrogram ve genetik uzaklık matrisi elde edilmiştir. İncelenen 96 genotipte
RAPD markör analizinde en düşük genetik uzaklık değeri 0.05, en yüksek genetik uzaklık değeri 0.84 olarak
tesbit edilmiştir. AFLP markör analizinde popülasyonda genetik uzaklık bakımından en düşük değer 0.15, en
yüksek değer 0.71’dir. SSR markör analizinde ise en düşük ve en yüksek genetik uzaklık değerleri ise 0.00 ile
0.87 olarak saptanmıştır. RAPD, AFLP ve SSR markör analizi verileri birlikte değerlendirilerek elde edilen
genetik uzaklık matrisinde, en düşük değer 0.14, en yüksek değer ise 0.70 olarak belirlenmiştir.
Characterization of Olive Genotypes (Olea europaea L.) by Means of DNA Markers
In this study, 96 genotypes in the National Ex-situ Olive Germplasm Bank have been characterized by DNAbased marker techniques such as RAPD, AFLP and SSR. In the marker analyses, 215 polymorphic bands from 52
primers in RAPD, 919 polymorphic bands from 26 primers in AFLP and 62 polymorphic bands from 14 primers
in SSR have been obtained. A dendrogram and a genetic distance matrix have been established with the data of
each technique seperately. Besides, the dendrogram and the genetic distance matrix have also been constructed by
evaluating totaly 1196 polymorphic bands as a result of combining the data of these techniques. In the studied 96
genotypes, it has been determined that the lowest genetic distance value was 0.05 and the highest genetic distance
value was 0.84 in RAPD marker analysis. In AFLP marker analysis, the lowest value was 0.15 and the highest
value was 0.71 within the population in the context of genetic distance. As for SSR, the lowest and highest
genetic distance values have been determined as 0.00 and 0.87 respectively. Evaluating the data of RAPD, AFLP
and SSR marker analyses together, it has been determined that the lowest genetic matrix value was 0.14 and the
highest genetic matrix value was 0.70.
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- ISSN: 1309-5889
- Başlangıç: 2010
- Yayıncı: Zeytincilik Araştırma Enstitüsü Müdürlüğü - İZMİR
Sayıdaki Diğer Makaleler
Gemlik Zeytin Çeşidinin Yarı Odun Çeliklerinin Köklendirilmesi
Zerbab GÜLER, Mücahit Taha ÖZKAYA, Saeed DOUSTİ
Zeytin (Olea europaea L.) Genotiplerinin DNA Markörleri Yardımı ile Karakterizasyonu
Öznur ÇETİN, Adalet MISIRLI, M. Bahattin TANYOLAÇ
Dilek ÖZDOĞAN, Renan TUNALIOĞLU
Türkiye ve Dünyada Sık Dikim Zeytin Yetiştiriciliği